Thermo Fisher Scientific Applied Biosystems Power SYBR Green PCR Master Mix

Thermo Fisher Scientific Applied Biosystems Power SYBR Green PCR Master Mix

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Product Details:

Accuracy High accuracy for DNA quantitation and gene expression
Usage Type Laboratory reagent
Shelf Life 12 months when properly stored
Instruments Type Compatible with Applied Biosystems Real-Time PCR systems
Storage Instructions Store at -20°C, Protect from light
Features High sensitivity, Broad dynamic range, Inhibitor tolerance, ROX passive reference included, Ready-to-use mix, Optimized for Applied Biosystems instruments
Function DNA amplification and detection
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Thermo Fisher Scientific Applied Biosystems Power SYBR Green PCR Master Mix Price And Quantity

Thermo Fisher Scientific Applied Biosystems Power SYBR Green PCR Master Mix Product Specifications

Accuracy High accuracy for DNA quantitation and gene expression
Dimension (L*W*H) Not Applicable (Supplied in reagent bottles/tubes)
Technology SYBR Green I dye-based real-time PCR
Equipment Type PCR Master Mix
Real-Time Operation Yes
Portable Yes (Supplied in small reagent bottles/tubes)
Condition New
Instruments Type Compatible with Applied Biosystems Real-Time PCR systems
Shelf Life 12 months when properly stored
Usage Type Laboratory reagent
Storage Instructions Store at -20°C, Protect from light
Operating Type Manual, compatible with Real-Time PCR systems
Wall Mounted No
Material Ready-to-use liquid formulation
Measurement Range Broad range, sensitive down to low copy numbers
Features High sensitivity, Broad dynamic range, Inhibitor tolerance, ROX passive reference included, Ready-to-use mix, Optimized for Applied Biosystems instruments
Function DNA amplification and detection
Color Green (contains SYBR Green dye)
Use Quantitative PCR (qPCR), gene expression analysis, DNA quantitation

Enzyme Hot start Taq DNA Polymerase
Product Code 4367659
Passive Reference Dye ROX included
Volume per Reaction Recommended 20 µL total reaction volume
Shipping Condition Ships on dry ice
Application Gene expression studies, genetic variation analysis, pathogen detection
Buffer Proprietary optimized PCR buffer
Form Liquid

Product Description

Brand	Thermo Fisher Scientific
Product Type	Master Mix
Pack Size	10 x 5 mL
CAT No	4368708
Application	Molecular Biology

Get superior nucleic acid quantitation with Applied Biosystems Power SYBR Green Master Mix. Power SYBR Master Mix offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

Optimized for Performance and Convenience

This SYBR Green PCR Master Mix is specially formulated for accuracy and ease of use in high-throughput laboratories. Its liquid, ready-to-use design eliminates the need for extensive reagent preparation, while delivering reliable amplification and detection of DNA targets with minimal hands-on time.

Broad Applications in Genomics and Diagnostics

The master mix supports a variety of real-time PCR experiments, including gene expression profiling, genetic variation analysis, and rapid pathogen detection. Its high sensitivity and wide dynamic range allow detection of low copy number targets, enhancing research precision in multiple fields.

Advanced Chemistry for Reliable Results

Equipped with hot start Taq DNA polymerase and proprietary optimized buffer, this reagent ensures specificity and consistent performance even in challenging conditions. The inclusion of ROX passive reference dye further stabilizes fluorescence readings on compatible Applied Biosystems real-time PCR platforms.

FAQ's of Thermo Fisher Scientific Applied Biosystems Power SYBR Green PCR Master Mix:

Q: How should the Power SYBR Green PCR Master Mix be stored for optimal stability?

A: To maintain reagent quality, store the Power SYBR Green PCR Master Mix at -20C and protect it from light. When handled properly, it retains full activity for up to 12 months.

Q: What is the recommended reaction setup for this master mix?

A: It is recommended to use a total reaction volume of 20 L per well or tube. Simply add your template DNA and primers to the ready-to-use liquid mix, minimizing setup errors and variability.

Q: When is it appropriate to use this master mix in PCR workflows?

A: This mix is ideal for quantitative PCR (qPCR), DNA quantitation, gene expression analysis, and genetic variation studies-especially when high sensitivity and inhibitor tolerance are required.

Q: Where can I use the Power SYBR Green PCR Master Mix?

A: Designed for use in research and diagnostics laboratories, this master mix is compatible with all Applied Biosystems Real-Time PCR systems and is suitable for both small-scale and high-throughput workflows.

Q: What are the main benefits of using this particular PCR master mix?

A: Key benefits include high sensitivity, broad measurement range, inhibitor tolerance, and accurate quantitation. Its ready-to-use format and ROX reference dye ensure optimized performance on Applied Biosystems qPCR systems.

Q: How does the mix ensure accuracy during real-time PCR analysis?

A: The inclusion of SYBR Green I dye allows precise detection of amplified DNA, while ROX passive reference dye normalizes signal variation, resulting in highly accurate and reproducible results.

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