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Thermo Fisher Scientific Invitrogen Quant-iT dsDNA Assay Kits, high sensitivity (HS) and broad range (BR) Thermo Fisher Scientific Invitrogen Quant-iT dsDNA Assay Kits, high sensitivity (HS) and broad range (BR) Thermo Fisher Scientific Invitrogen Quant-iT dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)
Thermo Fisher Scientific Invitrogen Quant-iT dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)
Thermo Fisher Scientific Invitrogen Quant-iT dsDNA Assay Kits, high sensitivity (HS) and broad range (BR) Thermo Fisher Scientific Invitrogen Quant-iT dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)

Thermo Fisher Scientific Invitrogen Quant-iT dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)

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Product Details:

  • Storage Instructions Store at 28C; protect from light
  • Measurement Range HS: 0.2 ng to 100 ng; BR: 2 ng to 1000 ng (per sample)
  • Usage Type Laboratory/Research Use Only
  • Shelf Life See kit label; typically 6-12 months
  • Accuracy HS kit typically accurate from 10 pg/L to 100 ng/L; BR kit from 100 pg/L to 1000 ng/L
  • Display Type Not Applicable (readout via fluorometer)
  • Instruments Type Reagent kit for use with compatible fluorescence microplate readers or fluorometers
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Thermo Fisher Scientific Invitrogen Quant-iT dsDNA Assay Kits, high sensitivity (HS) and broad range (BR) Price And Quantity

Thermo Fisher Scientific Invitrogen Quant-iT dsDNA Assay Kits, high sensitivity (HS) and broad range (BR) Product Specifications

  • Not Applicable (readout via fluorometer)
  • HS kit typically accurate from 10 pg/L to 100 ng/L; BR kit from 100 pg/L to 1000 ng/L
  • Fluorescence-based Quantification
  • Not Applicable (Used with compatible fluorometer)
  • See kit label; typically 6-12 months
  • New
  • Detects and quantifies double-stranded DNA
  • Kit box: White with blue and orange labeling; reagents: colorless/clear
  • High sensitivity (HS) and broad range (BR); specific for dsDNA; rapid results; compatible with multiple fluorometers
  • Yes (kit format)
  • Manual (Microplate/Tube Format)
  • No
  • Reagent kit for use with compatible fluorescence microplate readers or fluorometers
  • Approx. <1 kg per kit (varies by kit size)
  • HS: 0.2 ng to 100 ng; BR: 2 ng to 1000 ng (per sample)
  • Store at 28C; protect from light
  • Quantitative measurement of double-stranded DNA (dsDNA)
  • No
  • Kit packaging: Standard small reagent kit box
  • Laboratory/Research Use Only
  • Reagents and dyes in solution
  • DNA Quantification Assay Kit

Product Description

Brand Thermo Fisher Scientific
Product Type dsDNA Assay Kit
Pack Size 4 to 1000 ng
CAT No Q33130
Application Molecular Biology

Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and the Quant-iT Broad-Range dsDNA Assay Kit provide concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. These DNA assay kits are highly selective for double-stranded DNA over RNA, and, in the ranges of 0.2a100 ng DNA (for the HS dsDNA Assay Kit) and 4a1000 ng DNA (for the BR dsDNA Assay Kit), the fluorescence signal is linear.



Sensitive and Versatile DNA Quantification

Quant-iT dsDNA Assay Kits deliver highly accurate quantification for double-stranded DNA across a broad dynamic range. The High Sensitivity (HS) kit detects low-abundance samples, while the Broad Range (BR) kit is ideal for concentrated DNA preparations. Both are compatible with Qubit fluorometers and microplate readers, ensuring flexibility and ease in sample processing for a wide spectrum of molecular biology research needs.


Rapid Results with Simple Workflow

These assay kits are designed for efficiency, providing quantitative dsDNA data in as little as 2-5 minutes after reagent addition. Minimal sample preparation and straightforward protocols enable high-throughput workflows, supporting 100, 500, or 1000 reactions per kit. A standard DNA calibrator-essential for precise measurements-is included, streamlining the calibration process.


Reliable Reagents, Trusted Performance

Built with meticulously formulated dyes and reagents, each kit guarantees specificity for double-stranded DNA, minimizing interference from RNA or single-stranded DNA. Traceability is ensured by unique lot and catalog numbers. Packaged compactly, the kits are easily portable within the laboratory and suited for research use only. Proper storage under refrigeration and protection from light safeguard the integrity and shelf life of the reagents.

FAQ's of Thermo Fisher Scientific Invitrogen Quant-iT dsDNA Assay Kits, high sensitivity (HS) and broad range (BR):


Q: How do I choose between the High Sensitivity (HS) and Broad Range (BR) Quant-iT dsDNA Assay Kits?

A: Select the HS kit for quantifying low concentrations of dsDNA (as low as 10 pg/L, up to 100 ng/L per sample) and the BR kit for higher DNA amounts (100 pg/L to 1000 ng/L, or 2 ng to 1000 ng per sample). Match your expected sample concentration to the kit's measurement range for optimal accuracy.

Q: What types of samples are compatible with these dsDNA assay kits?

A: The kits are designed to work with purified DNA, PCR products, genomic DNA, plasmids, and other double-stranded DNA samples. They should not be used directly with crude cell lysates or samples high in contaminants, as this may affect accuracy.

Q: What is the process to use the Quant-iT dsDNA Assay Kit?

A: Simply mix your DNA sample with the assay reagent and standard DNA in the recommended assay volume (typically 200 L). After a brief incubation of about 2-5 minutes, measure the fluorescence using a compatible instrument (Qubit fluorometer or microplate reader with ex/em 485 nm/530 nm filters). Use the standard curve for quantification.

Q: Can I use the Quant-iT dsDNA kits with microplate readers?

A: Yes, both HS and BR kits are fully compatible with microplate readers as long as your reader can excite and detect at 485 nm (excitation) and 530 nm (emission). Ensure proper calibration with the provided DNA standards for reliable results.

Q: What are the storage and shelf life requirements for these kits?

A: Store all components at 2-8C and protect them from light to maintain reagent stability. Shelf life is typically 6-12 months from the date of manufacture; refer to the kit label for exact expiration details.

Q: How do I identify the lot and catalog numbers of my kit for traceability?

A: Both the lot and catalog numbers are clearly printed on the kit box and on reagent bottles. This information is crucial for quality control and for communicating with suppliers or technical support.

Q: What are the key benefits of using Quant-iT dsDNA Assay Kits?

A: These kits deliver high sensitivity and broad measurement range, require minimal hands-on time, and provide specific, reliable quantification of dsDNA. They are ideal for labs needing rapid, accurate DNA quantification in purified samples, and are portable, easy to store, and compatible with widely used laboratory instruments.

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