Qiagen Puregene Tissue Kit (33 g)

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Product Details:

Shelf Life 12 months
Instruments Type Life Science Laboratory Instruments
Usage Type Laboratory, Research
Storage Instructions Store at room temperature (1525C)
Function DNA Isolation
Accuracy High yield and purity
Measurement Range Various animal tissues
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Qiagen Puregene Tissue Kit (33 g) Price And Quantity

Qiagen Puregene Tissue Kit (33 g) Product Specifications

Real-Time Operation No
Shelf Life 12 months
Storage Instructions Store at room temperature (1525C)
Noise Level Silent Operation
Condition New
Color White (packaging and contents)
Operating Type Manual
Use Genomic DNA Purification from Animal Tissues
Function DNA Isolation
Technology Silica Membrane-Based Purification
Measurement Range Various animal tissues
Accuracy High yield and purity
Equipment Type DNA Purification Kit
Usage Type Laboratory, Research
Features High-purity DNA, scalable sample input, ready-to-use reagent mix
Material Reagents and Enzyme Mixtures
Instruments Type Life Science Laboratory Instruments
Weight 33 g (kit contents)
Wall Mounted No
Portable Yes

Product Description

Brand Qiagen

Product Type PCR Kits

Pack Size 33g

CAT No 158066

Application Molecular Reagent

Puregene Kits enable purification of high-molecular-weight (100 200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows samples to be purified for use as long-term references.

Efficient DNA Isolation for Molecular Research

The Qiagen Puregene Tissue Kit offers streamlined genomic DNA extraction from a variety of animal tissues. Its advanced silica membrane-based technology ensures consistently high purity and yield, supporting reliable results for molecular biology and genetic research. Ideal for both routine and demanding laboratory workflows.

Flexible and Scalable Sample Handling

Designed to process 10-20 mg samples per extraction, this kit is suitable for both small- and large-scale projects. Its reagent mixes are ready to use and adaptable, allowing laboratories to adjust workflow volumes based on research needs, maximizing productivity and convenience.

User-Friendly Workflow with Quick Turnaround

With an approximate protocol time of 60 minutes per sample, the kit minimizes hands-on time. Its straightforward, manual process requires no special instrumentation. Clear instructions and simplified steps enable seamless integration even in busy laboratory environments.

FAQ's of Qiagen Puregene Tissue Kit (33 g):

Q: How do I use the Qiagen Puregene Tissue Kit for DNA extraction?

A: Begin by lysing 10-20 mg of fresh or frozen animal tissue with the provided reagents. Follow the manual protocol, which involves binding DNA to a silica membrane, washing to remove impurities, and eluting purified DNA in 50-200 l of buffer. The kit contains all necessary reagents for these steps.

Q: What sample types are compatible with this tissue kit?

A: The kit is optimized for fresh or frozen animal tissues. It accommodates a variety of animal specimens, making it suitable for diverse genomic applications and research studies.

Q: When should I use this kit in my molecular biology workflow?

A: Use the Puregene Tissue Kit whenever high-yield, high-purity genomic DNA is required for downstream applications such as PCR, sequencing, or genetic studies.

Q: Where should the kit be stored, and what is its shelf life?

A: Store the Puregene Tissue Kit at room temperature (15-25C). The kit maintains its full functionality and reagent stability for up to 12 months from the date of manufacture.

Q: What is the benefit of silica membrane technology in this kit?

A: The silica membrane provides efficient binding and purification of DNA, ensuring high yield and purity with minimal contaminant carryover. This results in reliable, reproducible DNA suitable for sensitive molecular analyses.

Q: How many extractions can I perform with the 33 g kit size?

A: The 33 g kit provides sufficient reagents for multiple DNA extractions, depending on sample input and elution volumes, making it cost-efficient for research labs of various sizes.

Q: What are the main applications of DNA isolated with this kit?

A: Purified DNA is suitable for PCR, sequencing, molecular biology workflows, and genetic studies. The kit's high-quality output supports accurate and robust results in a range of downstream applications.

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