Thermo Fisher Scientific Applied Biosystems PowerUp SYBR Green Master Mix for qPCR

Thermo Fisher Scientific Applied Biosystems PowerUp SYBR Green Master Mix for qPCR

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Product Details:

Usage Type Laboratory reagent for research use only
Function Real-Time PCR amplification and quantitative analysis
Instruments Type Real-Time PCR (qPCR) system
Features Room temperature stability, hot-start activation, sensitive detection, broad instrument compatibility, improved specificity, pre-mixed format
Accuracy High accuracy for DNA quantification and detection
Shelf Life 12 months (when stored as recommended)
Measurement Range Dynamic detection over a wide range of DNA concentrations
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Thermo Fisher Scientific Applied Biosystems PowerUp SYBR Green Master Mix for qPCR Price And Quantity

Supplier Thermo Fisher Scientific
Sample Input For use with DNA or cDNA template
Enzyme Dual-Lock Taq DNA Polymerase (hot-start)
Compatibility Compatible with most standard and fast qPCR instruments
Application Gene expression analysis, DNA quantification, genetic variation studies, high-throughput applications
Format 2X ready-to-use master mix
Product Name Applied Biosystems PowerUp SYBR Green Master Mix
Number of Reactions Available in different sizes (e.g., 100, 500, 5,000 reactions depending on packaging)
Stability Stable for up to 24 hours at room temperature during setup

Thermo Fisher Scientific Applied Biosystems PowerUp SYBR Green Master Mix for qPCR Product Specifications

Operating Type Automated, for use with compatible qPCR instruments
Measurement Range Dynamic detection over a wide range of DNA concentrations
Material Nucleic acid reagents, including SYBR Green dye, DNA polymerase, dNTPs, and reaction buffer
Dimension (L*W*H) Not Applicable (supplied as a liquid reagent in vials)
Storage Instructions Store at -20C; avoid repeated freeze-thaw cycles
Wall Mounted No
Instruments Type Real-Time PCR (qPCR) system
Function Real-Time PCR amplification and quantitative analysis
Features Room temperature stability, hot-start activation, sensitive detection, broad instrument compatibility, improved specificity, pre-mixed format
Shelf Life 12 months (when stored as recommended)
Accuracy High accuracy for DNA quantification and detection
Use Quantitative PCR amplification and detection of DNA targets
Usage Type Laboratory reagent for research use only
Technology Real-Time PCR (qPCR)
Real-Time Operation Yes
Equipment Type qPCR Master Mix
Portable Yes (vial/kit format)
Color Green (SYBR Green dye) in clear liquid formulation
Condition New

Supplier Thermo Fisher Scientific
Sample Input For use with DNA or cDNA template
Enzyme Dual-Lock Taq DNA Polymerase (hot-start)
Compatibility Compatible with most standard and fast qPCR instruments
Application Gene expression analysis, DNA quantification, genetic variation studies, high-throughput applications
Format 2X ready-to-use master mix
Product Name Applied Biosystems PowerUp SYBR Green Master Mix
Number of Reactions Available in different sizes (e.g., 100, 500, 5,000 reactions depending on packaging)
Stability Stable for up to 24 hours at room temperature during setup

Product Description

Brand	Thermo Fisher Scientific
Product Type	Master Mix
Pack Size	10 x 5 mL
CAT No	A25778
Application	Molecular Biology

PowerUp SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. Coupled with user-supplied primer sets and template, PowerUpa SYBRa Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.

Achieve Reliable qPCR Results

The PowerUp SYBR Green Master Mix streamlines your qPCR workflow. Its advanced Dual-Lock Taq DNA Polymerase ensures precise hot-start activation and reduces non-specific amplification. The pre-mixed 2X formulation accelerates setup and reduces handling errors, supporting high-quality results in quantitative DNA analysis. Room temperature stability during setup adds flexibility to your lab routines.

Compatible with Most qPCR Instruments

Designed for broad compatibility, this master mix works seamlessly with the majority of standard and fast real-time PCR instruments. Whether your research utilizes standard protocols or high-throughput screening, the mix's robustness and sensitivity remain uncompromised, ensuring reliable performance across platforms.

FAQ's of Thermo Fisher Scientific Applied Biosystems PowerUp SYBR Green Master Mix for qPCR:

Q: How should I store the Applied Biosystems PowerUp SYBR Green Master Mix for maximum shelf life?

A: For optimal stability, store the master mix at -20C. Avoid repeated freeze-thaw cycles and, during setup, the mix remains stable at room temperature for up to 24 hours.

Q: What types of samples can be used with this qPCR master mix?

A: The PowerUp SYBR Green Master Mix is suitable for use with either DNA or cDNA templates, making it adaptable for diverse gene expression, quantification, and genetic variation studies.

Q: When should the master mix be used within its shelf life?

A: The mix should be used within 12 months of purchase, provided it has been stored as recommended. Always check the expiration date on the packaging before beginning your experiment.

Q: Where can this master mix be applied in research workflows?

A: It is ideal for quantitative gene expression analysis, DNA target quantification, genetic variation studies, as well as high-throughput screening and detection projects in research laboratories.

Q: What benefits does the Dual-Lock Taq DNA Polymerase provide during qPCR?

A: The hot-start Dual-Lock Taq DNA Polymerase provides enhanced specificity and sensitivity by preventing premature enzyme activity, leading to more accurate amplification and reduced non-specific products.

Q: How many qPCR reactions can be performed with a single package?

A: The master mix is available in a variety of packaging sizes, suitable for 100, 500, or up to 5,000 reactions, depending on your experimental scale and needs.

Q: What makes this master mix suitable for high-throughput applications?

A: The pre-mixed format, high accuracy, robust performance across instruments, and stability at room temperature make it highly suitable for high-throughput and automated laboratory settings.

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