Qiagen EpiTect Fast 96 FFPE Bisulfite Kit

Qiagen EpiTect Fast 96 FFPE Bisulfite Kit

Get a Price/Quote

Product Details:

Shelf Life 12 months
Storage Instructions Store at 2-8C
Measurement Range 12 g DNA per reaction
Accuracy High conversion accuracy (>99%)
Features Rapid processing time; optimized for FFPE DNA; high conversion efficiency
Function Bisulfite conversion of unmethylated cytosines for methylation analysis
Instruments Type Molecular Analysis Kit
Click to View more
Share Your Product:

Request to Call Back Send Inquiry

Qiagen EpiTect Fast 96 FFPE Bisulfite Kit Price And Quantity

Qiagen EpiTect Fast 96 FFPE Bisulfite Kit Product Specifications

Features Rapid processing time; optimized for FFPE DNA; high conversion efficiency
Dimension (L*W*H) Standard 96-well plate format
Function Bisulfite conversion of unmethylated cytosines for methylation analysis
Instruments Type Molecular Analysis Kit
Wall Mounted No
Shelf Life 12 months
Measurement Range 12 g DNA per reaction
Accuracy High conversion accuracy (>99%)
Material Plastic, chemical reagents
Operating Type Manual/Automated compatible
Portable Yes
Storage Instructions Store at 2-8C
Real-Time Operation Yes, compatible with real-time PCR
Usage Type Laboratory
Noise Level Silent
Color White/transparent
Weight Approx. 0.5 kg
Equipment Type DNA Bisulfite Conversion Kit
Use DNA methylation analysis
Condition New
Technology Fast bisulfite conversion of DNA from FFPE samples

Product Description

Brand Qiagen

Product Type PCR Kits

Pack Size 96 Wells

CAT No 59740

Application Molecular Reagent

EpiTect Fast Bisulfite Conversion Kits are a coordinated system of kits that enable highly efficient conversion of unmethylated cytosines to uracils in as little as 30 minutes while offering outstanding flexibility in choice of starting material. Streamlined procedures for sample lysis and direct bisulfite conversion of DNA from whole blood, cultured cells, tissue samples, genomic DNA and formalin-fixed, paraffin-embedded (FFPE) slices make efficient bisulfite conversion fast and effortless. Innovative DNA Protect technology ensures effective DNA denaturation without degradation, resulting in high DNA yield and reproducible results. The spin-column procedures can be automated on theQIAcube Connect. The procedure in 96-well format delivers ultrafast, high-throughput bisulfite conversion of DNA and can be processed using a centrifuge or vacuum manifold.

Optimized for FFPE Samples

This kit is specifically tailored for DNA extracted from FFPE tissue, ensuring optimal yield and conversion results. By addressing the unique challenges presented by FFPE samples, such as degraded or cross-linked DNA, the kit produces high-quality bisulfite-converted DNA ideal for sensitive methylation analyses.

Fast, Scalable Workflow

Utilize the 96-well plate format to process up to 96 samples in just 90 minutes, dramatically accelerating your methylation studies. The kit seamlessly integrates with both automated platforms, like QIAcube, and manual procedures, enabling high-throughput operations in any lab setting.

High Conversion Accuracy and Quality

With greater than 99% cytosine conversion efficiency, the kit ensures that results from downstream analyses such as qPCR and sequencing are highly accurate. This reliability is critical for epigenetic research and diagnostics, providing confidence in your methylation data outputs.

FAQ's of Qiagen EpiTect Fast 96 FFPE Bisulfite Kit:

Q: How does the EpiTect Fast 96 FFPE Bisulfite Kit process DNA samples?

A: The kit utilizes a fast bisulfite treatment protocol optimized for DNA from FFPE tissue in a 96-well plate format, allowing simultaneous processing of up to 96 samples with high conversion efficiency.

Q: What is the main benefit of using this kit for FFPE tissue samples?

A: It effectively converts unmethylated cytosines to uracil in DNA from challenging FFPE tissue, resulting in high-quality DNA suitable for methylation analysis with minimal sample loss and high accuracy.

Q: When should I use this bisulfite conversion kit in my workflow?

A: This kit should be used prior to downstream methylation analyses, such as qPCR or sequencing, immediately after isolating DNA from FFPE tissue samples.

Q: Where can the kit be operated-manually or in automated systems?

A: The kit is compatible with both manual workflows and automated platforms, including QIAcube, offering flexibility based on your laboratory's needs.

Q: What is the typical processing time and how much DNA should I use per reaction?

A: The recommended processing time is approximately 90 minutes, with an optimal DNA input range of 1-2 g per reaction, ensuring high conversion efficiency and yield.

Q: How should I store the EpiTect Fast 96 FFPE Bisulfite Kit and what is its shelf life?

A: Store the kit at 2-8C as per instructions. The product has a shelf life of 12 months under proper storage conditions.

Q: What downstream analyses is the bisulfite-converted DNA compatible with?

A: The converted DNA is suitable for various methylation analyses, including real-time PCR (qPCR) and sequencing applications, supporting high-sensitivity epigenetic studies.

Tell us about your requirement

Price:

Quantity

Select Unit

50
100
200
250
500
1000+

Additional detail

Mobile number

Other Products in 'Medical Reagents' category

Snibe MAGLUMI PRG (CLIA) Condition : Other, New, Unopened Technology : Chemiluminescence Immunoassay (CLIA) Use : Progesterone (PRG) Quantitative Detection in Serum/Plasma Usage Type : Laboratory Diagnostic Use Accuracy : High (based on analyzer calibration and kits reference standards) Instruments Type : CLIA Reagent Kit (for Snibe MAGLUMI series analyzers) Features : High Sensitivity, Specificity, Automation, Rapid Turnaround Function : Quantitative estimation of serum/plasma progesterone Color : Reagent Vials and Kit Components Standard Transparent/White Operating Type : Automatic Storage Instructions : Store at 28C, Avoid Freezing Wall Mounted : No Portable : No (to be used with compatible analyzer) Measurement Range : Determined by analyzer and assay calibrator; typically covers whole clinical range for progesterone Real-Time Operation : Yes Shelf Life : 12 to 18 months (as per batch label) Material : Other, Diagnostic Grade Reagents and Consumables Power Source : Electric Equipment Type : Automated Chemiluminescence Immunoassay Analyzer (CLIA) Kit Weight : Consummable use negligible weight Dimension (L*W*H) : Refer to analyzer specifications Noise Level : Low

Send Inquiry

Qiagen EZ1&2 DNA FFPE Kit (48) Condition : New Technology : Magnetic beadbased purification Use : Extraction of genomic DNA from formalinfixed, paraffinembedded (FFPE) tissues Usage Type : Laboratory research Accuracy : Yields DNA suitable for downstream molecular applications Instruments Type : EZ1 and EZ2 Advanced/Connect instruments Features : Automationready, optimized for FFPE DNA isolation, high reproducibility, minimal crosscontamination, compatible with Qiagen EZ1 and EZ2 instruments Function : Automated DNA isolation from FFPE samples Color : White/Transparent (reagents/columns) Operating Type : Other, Automatic (with EZ1/EZ2 instruments) Storage Instructions : Store at room temperature (1525C), protect from light Wall Mounted : No Portable : Yes (kit format) Measurement Range : Typical DNA yield: 15 g from 2 sections (10 m each; depends on sample) Real-Time Operation : No Shelf Life : 12 months from date of manufacture Material : Other, Proprietary chemical reagents and spin columns Power Source : Other, Not Applicable (used with powered EZ1/EZ2 instruments) Equipment Type : DNA Extraction Kit Weight : ~1.1 kg (kit box) Dimension (L*W*H) : Kit box dimensions: ~23 x 20 x 13 cm (approximate) Noise Level : Silent/Not Applicable

Send Inquiry

Qiagen QIAseq FX DNA Library Kit HT A-D (384) Condition : New Technology : Enzymatic Fragmentation and Library Prep (NGS) Use : Preparation of DNA libraries for NextGeneration Sequencing Usage Type : Laboratory use Accuracy : High fidelity, minimizes sequence bias Instruments Type : NGS Library Preparation Kit Features : Highthroughput, enzymatic fragmentation, rapid protocol, high yield, compatible with Illumina platforms Function : Simultaneous DNA fragmentation and library prep Color : Multicolor (as per reagent) Operating Type : Manual Storage Instructions : Store at 20C Wall Mounted : No Portable : Yes Measurement Range : Compatible with DNA input 10 ng 1 g Real-Time Operation : No Shelf Life : 12 months from manufacturing date Material : Other, Reagents, Enzyme Mix, Buffers, Solution, Tubes Power Source : Other, Not required (compatible with common laboratory thermal cycler) Equipment Type : DNA Library Preparation Kit Weight : Approx. 1 kg (shipping weight) Dimension (L*W*H) : Kit dimensions vary; standard laboratory kit size

Send Inquiry

Qiagen QuantiNova LNA PCR Assay (750) Condition : New Technology : Locked Nucleic Acid (LNA) enhanced realtime PCR Use : Quantitative detection of nucleic acids in research applications Usage Type : Research Use Only (RUO) Accuracy : High accuracy, dependent on protocol adherence Instruments Type : Compatible with standard realtime PCR cyclers Features : High specificity and sensitivity for target detection, LNAmodified primers and probes Function : Quantitative PCR (qPCR) detection Color : Kit components: typically clear or colorless Operating Type : Other, Automated PCR based Storage Instructions : Store at 20C Wall Mounted : No Measurement Range : Wide dynamic range suitable for low and high copy number targets Real-Time Operation : Yes Shelf Life : 12 months (when stored properly) Power Source : Other, Not applicable (consumable; used with PCR instruments) Equipment Type : PCR Assay Kit

Send Inquiry

SEND INQUIRY CALL ME FREE