|
Thermo Fisher Scientific Applied Biosystems Absolute Q 1-step RT-dPCR Master Mix (4X)
Product Details:
- Usage Type Laboratory reagent
- Shelf Life 12 months
- Measurement Range Dependent on platform and assay design
- Features 1-step RT-dPCR, high sensitivity, optimized for absolute quantification, compatible with the Absolute Q Platform
- Accuracy High, suitable for precise quantification
- Storage Instructions Store at -20C; avoid repeated freeze-thaw cycles
- Function Facilitates cDNA synthesis and dPCR amplification in a single tube
- Click to View more
X
Thermo Fisher Scientific Applied Biosystems Absolute Q 1-step RT-dPCR Master Mix (4X) Price And Quantity
- Typically used in standard dPCR reaction volumes (consult protocol)
- RNase- and DNase-free
- Supplied in 1 mL tube
- A43252
- For Research Use Only. Not for use in diagnostic procedures.
- Suitable for high-throughput applications
- Thermostable reverse transcriptase and DNA polymerase included
- -20°C
- Designed specifically for Applied Biosystems Absolute Q Digital PCR Platform
- Requires gentle mixing before use
- Viral load, gene expression, mutation detection, copy number variation
- QC tested for dPCR performance and sensitivity
- 4X concentrated master mix
Thermo Fisher Scientific Applied Biosystems Absolute Q 1-step RT-dPCR Master Mix (4X) Product Specifications
- Facilitates cDNA synthesis and dPCR amplification in a single tube
- Dependent on platform and assay design
- No
- Liquid reagent formulation
- 12 months
- Laboratory reagent
- One-step reverse transcription and digital PCR amplification
- No
- Manual pipetting or automated liquid handling
- Store at -20C; avoid repeated freeze-thaw cycles
- New
- RT-dPCR (Reverse Transcription Digital PCR)
- Master Mix for Digital PCR (dPCR)
- N/A (supplied in reagent tubes/bottles)
- Not required (used in thermocyclers)
- Consumable reagent for digital PCR systems
- Clear/Colorless liquid
- Yes, enables real-time RT-dPCR
- 1-step RT-dPCR, high sensitivity, optimized for absolute quantification, compatible with the Absolute Q Platform
- High, suitable for precise quantification
- Typically used in standard dPCR reaction volumes (consult protocol)
- RNase- and DNase-free
- Supplied in 1 mL tube
- A43252
- For Research Use Only. Not for use in diagnostic procedures.
- Suitable for high-throughput applications
- Thermostable reverse transcriptase and DNA polymerase included
- -20°C
- Designed specifically for Applied Biosystems Absolute Q Digital PCR Platform
- Requires gentle mixing before use
- Viral load, gene expression, mutation detection, copy number variation
- QC tested for dPCR performance and sensitivity
- 4X concentrated master mix
Product Description
| Brand | Thermo Fisher Scientific |
| A30872 | Master Mix |
| Pack Size | 200 reactions |
| CAT No | A55146 |
| Application | Molecular Biology |
Applied Biosystems Absolute Q 1-step RT-dPCR Master Mix (4X) is optimized for use with the QuantStudio Absolute Q Digital PCR System in a simple workflow with minimal processing steps. The 4X formulation enables analysis of higher sample volumes and facilitates accurate quantification of RNA targets without using a standard curve.
Streamlined One-Step RT-dPCR Workflow
The Absolute Q 1-step RT-dPCR Master Mix simplifies viral RNA or gene expression analysis by combining reverse transcription and digital PCR amplification into a unified workflow. This eliminates the need for separate cDNA synthesis, reducing hands-on time and potential errors. Its 4X concentration allows direct incorporation of samples into standard digital PCR protocols on the Applied Biosystems Absolute Q Platform, ensuring rapid, reliable data output.
High Sensitivity and Precision in Quantification
This master mix is engineered for high accuracy and sensitivity in low-copy target detection, enabling precise quantification of viral loads, mutations, or gene expression. Advanced enzyme components and rigorous quality control ensure reproducible results, making it suitable for challenging research applications requiring absolute quantification and robust performance.
Optimized Compatibility and Easy Handling
Designed exclusively for use with the Absolute Q Digital PCR Platform, this reagent offers seamless compatibility and superior performance. Ready-to-use and supplied as a colorless liquid, it requires only gentle mixing before use. Packaged in a convenient 1 mL format, it is well-suited for both manual pipetting and automated liquid handling workflows in research laboratories.
FAQ's of Thermo Fisher Scientific Applied Biosystems Absolute Q 1-step RT-dPCR Master Mix (4X):
Q: How do I use the Applied Biosystems Absolute Q 1-step RT-dPCR Master Mix in my digital PCR experiments?
A: To use the master mix, combine it with your RNA sample and target-specific primers/probes according to the protocol for the Absolute Q Digital PCR Platform. Ensure gentle mixing before use, and run the reaction under recommended conditions to achieve simultaneous reverse transcription and digital PCR amplification in a single tube.Q: What are the key benefits of using this 1-step RT-dPCR master mix for my research applications?
A: This master mix streamlines workflows by enabling one-step cDNA synthesis and amplification, reducing handling time and contamination risk. Its high sensitivity and precision support accurate quantification of viral load, gene expression, and genetic variations in research experiments.Q: When should I use this master mix versus a standard dPCR or RT-PCR mix?
A: The 1-step RT-dPCR master mix is ideal when you require both reverse transcription and absolute quantification of RNA targets in a single reaction, as in viral load measurements or gene expression studies. Use standard dPCR or RT-PCR mixes if your application demands separate cDNA synthesis or uses non-Absolute Q platforms.Q: Where can I purchase this reagent in India, and in what format is it supplied?
A: You can obtain the Absolute Q 1-step RT-dPCR Master Mix through authorized Thermo Fisher Scientific dealers, distributors, exporters, manufacturers, suppliers, and wholesalers across India. It is provided as a 4X concentrated liquid in a 1 mL RNase- and DNase-free tube.Q: What storage and handling guidelines should I follow for this master mix?
A: Store the master mix at -20C and avoid repeated freeze-thaw cycles to maintain its optimal performance. Before using, allow the reagent to thaw completely and mix gently to ensure homogeneity.Q: How does the master mix maintain high accuracy and sensitivity during digital PCR?
A: The reagent contains thermostable reverse transcriptase and DNA polymerase enzymes that are QC tested for dPCR sensitivity and performance. Its optimized formulation ensures reliable detection of low-abundance targets with high reproducibility.Q: What types of research applications are supported by this product?
A: The master mix is suitable for a wide range of applications, including viral load quantification, gene expression analysis, mutation detection, and copy number variation studies, specifically on the Absolute Q Digital PCR Platform.Tell us about your requirement
Price:
Quantity
Select Unit
- 50
- 100
- 200
- 250
- 500
- 1000+
Additional detail
Mobile number
Email
Other Products in 'Medical Reagents' category
- Saaskin Corporation Private Limited.
GST : 33AAZCS6003M1ZU
- 275/184, 1st Floor, Office No: 2, Golden Enclave, Periyar E V R Salai, P.H. Road, Kilpauk,Chennai - 600010, Tamil Nadu, India
- Mobile : 0091 9840819191 / 9940116677
- Phone:07971189171
- Mr Rajesh H (Business Operations Manager)
- Mobile:07971189171
|
Send Inquiry |
SAASKIN CORPORATION PRIVATE LIMITED
All Rights Reserved.(Terms of Use) Developed and Managed by Infocom Network Private Limited. |
