Thermo Fisher Scientific Applied Biosystems TaqMan GTXpress Master Mix

Thermo Fisher Scientific Applied Biosystems TaqMan GTXpress Master Mix

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Product Details:

Measurement Range Wide dynamic range for template detection (110^6 copies)
Features Fast reaction times, high specificity, optimized for blockage-resistant detection, compatible with fast cycling protocols
Storage Instructions Store at -20C; avoid repeated freeze-thaw cycles
Usage Type Laboratory reagent
Shelf Life 12 months (when stored as recommended)
Function Real-time PCR amplification and detection
Instruments Type Compatible with Applied Biosystems real-time PCR systems
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Thermo Fisher Scientific Applied Biosystems TaqMan GTXpress Master Mix Price And Quantity

Thermo Fisher Scientific Applied Biosystems TaqMan GTXpress Master Mix Product Specifications

Real-Time Operation Yes
Measurement Range Wide dynamic range for template detection (110^6 copies)
Features Fast reaction times, high specificity, optimized for blockage-resistant detection, compatible with fast cycling protocols
Use Quantitative PCR (qPCR), gene expression analysis, genetic variation studies
Usage Type Laboratory reagent
Storage Instructions Store at -20C; avoid repeated freeze-thaw cycles
Color Clear solution
Technology TaqMan Probe-Based qPCR
Shelf Life 12 months (when stored as recommended)
Instruments Type Compatible with Applied Biosystems real-time PCR systems
Function Real-time PCR amplification and detection
Accuracy High accuracy in quantification of target nucleic acids
Equipment Type Real-Time PCR Master Mix
Operating Type Automatic
Portable Yes (provided in bottles/tubes for laboratory use)
Material Proprietary enzyme and buffer mix
Power Source Not applicable (used as reagent)
Condition New
Wall Mounted No

Reaction Volume Optimized for standard reaction volumes (1050 L)
Shipment Condition Ships on dry ice
Hot Start Taq DNA Polymerase Included (antibody-mediated) for increased specificity
Format 2X concentrated master mix
Catalog Number 4401892 (example, varies by pack size)
Compatible Sample Types Genomic DNA, cDNA, viral DNA/RNA (after reverse transcription)
Stability Stable for several hours at room temperature during setup

Product Description

Brand	Thermo Fisher Scientific
Product Type	Master Mix
Pack Size	10 mL
CAT No	4401892
Application	Molecular Biology

TaqMan GTXpress Master Mix delivers accurate genotyping results with robust performance in less than 50 min. Applied Biosystems TaqMan GTXpress Master Mix (2X) combines AmpliTaq Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX dye in a convenient single vial format. The 1 mL pack includes reagents sufficient to perform 400 PCR reactions (5 I14L/reaction).

Enhanced Specificity and Speed for qPCR

TaqMan GTXpress Master Mix incorporates antibody-mediated hot start Taq DNA polymerase, enabling superior specificity and fast cycling protocols. This allows users to perform real-time PCR reactions with reduced background and increased throughput, making it well suited for high-demand laboratories conducting genetic quantification and gene expression analysis.

Optimized Stability and Handling

The master mix is formulated to remain stable for several hours at room temperature during reaction setup, reducing concerns about reagent integrity. Its 12-month shelf life (when stored at -20C) ensures consistent performance, while shipment on dry ice preserves enzyme activity throughout transport to your laboratory.

Wide Application Range

Compatible with genomic DNA, cDNA, and viral DNA/RNA following reverse transcription, TaqMan GTXpress Master Mix supports diverse applications in molecular diagnostics and research. Its broad dynamic range (1-10^6 copies) facilitates accurate detection and quantification across a wide spectrum of target concentrations.

FAQ's of Thermo Fisher Scientific Applied Biosystems TaqMan GTXpress Master Mix:

Q: How do I use TaqMan GTXpress Master Mix for real-time PCR assays?

A: To use the TaqMan GTXpress Master Mix, simply prepare your reaction by mixing the 2X concentrated mix with your template DNA (genomic DNA, cDNA, or viral nucleic acids after reverse transcription), primers, and TaqMan probe in standard volumes (10-50 L). The mix is compatible with fast cycling protocols and Applied Biosystems real-time PCR instruments.

Q: What types of samples can be analyzed with this master mix?

A: The master mix is suitable for a variety of sample types, including genomic DNA, cDNA, and viral DNA or RNA (following reverse transcription), making it versatile for diverse genetic studies and diagnostic applications.

Q: When should I set up reactions after thawing the master mix?

A: After thawing from storage at -20C, you may set up your reactions within several hours at room temperature, as the mix is stable during this period. Avoid repeated freeze-thaw cycles to maintain reagent quality.

Q: Where should the TaqMan GTXpress Master Mix be stored when not in use?

A: Store the master mix at -20C when not in use. For optimal performance, ensure it is not subjected to repeated freezing and thawing.

Q: What benefits does this master mix provide in qPCR experiments?

A: This product offers high specificity due to its hot start Taq DNA polymerase, rapid reaction times, and resistance to common PCR inhibitors. Together, these features improve quantification accuracy and efficiency in gene expression and genetic variation studies.

Q: How is the TaqMan GTXpress Master Mix supplied and shipped?

A: The master mix is shipped on dry ice to maintain enzyme stability and is provided in clear solution bottles or tubes suitable for laboratory use. Different pack sizes are available under catalog number 4401892 (reference for example).

Q: Can I use this master mix with non-Applied Biosystems qPCR instruments?

A: While the master mix is optimized for Applied Biosystems real-time PCR systems, it may be compatible with other qPCR platforms. However, optimal results are ensured when used with recommended instruments.

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