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Thermo Fisher Scientific Invitrogen Quant-iT RNA Assay Kits and Quant-iT RNA HS Reagent Thermo Fisher Scientific Invitrogen Quant-iT RNA Assay Kits and Quant-iT RNA HS Reagent Thermo Fisher Scientific Invitrogen Quant-iT RNA Assay Kits and Quant-iT RNA HS Reagent
Thermo Fisher Scientific Invitrogen Quant-iT RNA Assay Kits and Quant-iT RNA HS Reagent
Thermo Fisher Scientific Invitrogen Quant-iT RNA Assay Kits and Quant-iT RNA HS Reagent Thermo Fisher Scientific Invitrogen Quant-iT RNA Assay Kits and Quant-iT RNA HS Reagent

Thermo Fisher Scientific Invitrogen Quant-iT RNA Assay Kits and Quant-iT RNA HS Reagent

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Product Details:

  • Shelf Life 12 months from date of manufacture
  • Usage Type Laboratory use, research applications
  • Function RNA quantification from various biological sources
  • Display Type Not Applicable (uses Qubit Fluorometer display)
  • Instruments Type Fluorescent nucleic acid quantification reagent
  • Features High sensitivity, low sample consumption, fast and easy workflow, compatibility with Qubit fluorometers, highly selective for RNA over DNA
  • Accuracy Accurate to within 10% in specified range
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Thermo Fisher Scientific Invitrogen Quant-iT RNA Assay Kits and Quant-iT RNA HS Reagent Price And Quantity

Thermo Fisher Scientific Invitrogen Quant-iT RNA Assay Kits and Quant-iT RNA HS Reagent Product Specifications

  • Laboratory use, research applications
  • Accurate quantification of RNA (total RNA, rRNA, mRNA) in research samples
  • Fluorometric quantitation using fluorescent dye
  • 12 months from date of manufacture
  • Yes, kit is portable
  • RNA quantification from various biological sources
  • Not Applicable (uses Qubit Fluorometer display)
  • No
  • Accurate to within 10% in specified range
  • High sensitivity, low sample consumption, fast and easy workflow, compatibility with Qubit fluorometers, highly selective for RNA over DNA
  • Proprietary reagent mixture, plastic assay tubes
  • Fluorescent nucleic acid quantification reagent
  • Approx. 400 g per kit (including packaging)
  • Reagents: Colorless to light yellow solution; Kit packaging: Standard Thermo Fisher branding
  • Manual pipetting, Compatible with Qubit fluorometer
  • Kit box size: Approx. 18 13 8 cm (for storage and handling)
  • No
  • New
  • Store at 28C (refrigerator), protect from light
  • Qubit RNA Assay Kit: 5 ng/mL to 100 ng/mL; Qubit RNA HS (High Sensitivity) Reagent: 250 pg/L to 100 ng/L
  • RNA Quantitation Assay Kit
  • Not Applicable (uses Qubit Fluorometer for detection)

Product Description

Brand Thermo Fisher Scientific
Product Type RNA Assay Kit
Pack Size 5 to 100 ng
CAT No Q32884
Application Molecular Biology

The Quant-iT RNA Reagent and Assay Kit makes RNA quantification easy and accurate. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. The assay is highly selective for RNA over double-stranded DNA, and provides a dynamic range of 5-100 ng of RNA.



High Sensitivity, Low Sample Consumption

With the Quant-iT RNA HS Reagent, researchers can accurately detect as little as 250 pg/L of RNA, ensuring even trace amounts are reliably measured. The efficient workflow is optimized for as little as 1 L of sample, preserving precious RNA from limited or precious samples. The reagent's selectivity for RNA over DNA further enhances data accuracy in RNA-specific workflows.


Fast and Easy Workflow for Research Labs

The Quant-iT RNA Assay Kits are engineered for simplicity and speed. The assay requires minimal hands-on time, with each reaction completed in approximately 2-5 minutes. The process involves combining sample, reagent, buffer, and standard in assay tubes, followed by fluorescence measurement using a Qubit Fluorometer. This streamlined approach supports high-throughput and reliable RNA quantification.


Broad Measurement Range and Reliable Standardization

These kits provide a robust dynamic range-5 ng/mL to 100 ng/mL for the Qubit RNA Assay Kit and 250 pg/L to 100 ng/L for the HS Reagent variant. Included are two ready-to-use RNA standards to enable accurate standard curve generation and quantitative results, ensuring repeatability and confidence in your measurements across the linear range.

FAQ's of Thermo Fisher Scientific Invitrogen Quant-iT RNA Assay Kits and Quant-iT RNA HS Reagent:


Q: How do I perform an RNA quantification assay with Invitrogen Quant-iT RNA Assay Kits?

A: To use the kits, add your RNA sample (1-20 L), RNA standard, quantitation reagent, and buffer into provided assay tubes. Mix gently, incubate for 2-5 minutes, and measure fluorescence on a compatible Qubit Fluorometer or other plate reader. Compare sample readings to standards to determine RNA concentration.

Q: What is the minimum sample volume and sensitivity for detection using these kits?

A: You can use as little as 1 L per assay. The Quant-iT RNA HS Reagent can reliably detect RNA concentrations as low as 250 pg/L, making it highly suited for low-concentration or precious RNA samples.

Q: Which instruments are compatible with the Quant-iT RNA Assay Kits?

A: The assays are designed for use with Qubit 2.0, 3.0, and 4 Fluorometers, but are also compatible with other plate readers featuring the appropriate fluorescence filters, broadening usability in various laboratory settings.

Q: What are the key benefits of using this RNA quantitation kit over traditional methods?

A: The main benefits include exceptional RNA selectivity (no significant DNA interference), rapid results (2-5 minutes per assay), low sample consumption, and straightforward, manual pipetting procedure, making it easier and faster than spectrophotometry or gel-based quantitation methods.

Q: How should I store the kit and what is its shelf life?

A: Store the kit at 2-8C in a refrigerator and protect all reagents from light to maintain stability. The kit remains effective for up to 12 months from the date of manufacture if stored properly.

Q: When and where should I use Quant-iT RNA Assay Kits?

A: These kits are intended exclusively for laboratory research use and are not suitable for diagnostic procedures. They are ideal for academic, pharmaceutical, and biotechnology research laboratories in need of accurate RNA quantification from cell lysates, tissues, or purified RNA samples.

Q: How is waste from this assay disposed of, and is the kit hazardous?

A: Dispose of used assay reagents and materials as per your local laboratory's chemical waste disposal regulations. The reagents are not classified as hazardous according to GHS, but standard laboratory safety protocols should be followed.

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