Product Description
Brand Qiagen
Product Type QIAamp
Pack Size 250T
CAT No 53706
Application Molecular Reagent
The QIAamp UltraSens Virus Kit uses a novel technology to concentrate viral nucleic acids in plasma and serum samples, followed by nucleic acid purification using proven QIAamp technology. Starting with sample volumes of up to 1 ml, nucleic acid concentration is achieved by first adding a novel reagent to the sample. The reagent forms complexes with nucleic acids, allowing them to be highly concentrated by low-speed centrifugation.
Ultra-Sensitive Detection Across Diverse VirusesHarnessing advanced silica-membrane technology, the Qiagen QIAamp UltraSens Virus Kit delivers superior sensitivity, facilitating the detection and quantification of even low-abundance viral RNA and DNA. This enables accurate viral diagnostics and monitoring for a range of infectious diseases directly from serum, plasma, or other cell-free body fluids.
Optimized Workflow for Consistent ResultsWith all necessary reagents and polypropylene spin columns included, the kit streamlines the manual nucleic acid extraction process. Designed for up to 250 preparations, it assures high reproducibility and efficiency, making it a reliable choice for routine laboratory workflows and clinical diagnostics.
Flexible, Secure, and User-Friendly OperationThe QIAamp UltraSens Virus Kit is portable and easy to use, requiring no specialized equipment beyond standard laboratory centrifugation. Compatible with the QIAcube for automation, and incorporating clear safety instructions for hazardous reagents, it is ideal for professional lab settings seeking both precision and convenience.
FAQ's of Qiagen QIAamp UltraSens Virus Kit (250):
Q: How do I use the Qiagen QIAamp UltraSens Virus Kit for viral RNA and DNA extraction?
A: The kit is used for manual purification of viral nucleic acids from up to 1 ml of serum, plasma, or other cell-free body fluids. Simply follow the supplied protocol to bind nucleic acids to the silica membrane, wash away contaminants, and elute purified RNA or DNA in 60-100 l.
Q: What types of viruses and samples are compatible with this kit?
A: This kit is suitable for a wide variety of viruses, allowing extraction from serum, plasma, and other cell-free body fluids. It supports both RNA and DNA viruses, even in samples with low viral titers.
Q: When should I use this kit in my workflow?
A: Utilize the kit when high-sensitivity viral detection, viral load monitoring, or infectious disease diagnosis is required. It is most effective in laboratory workflows demanding precise and reproducible nucleic acid purification.
Q: Where should the kit be stored and for how long can it be used?
A: Store the kit at 2-8C as directed on the label. Shelf life is typically 1-2 years from the date of manufacturing; always refer to the expiration date provided on the packaging.
Q: What is the process for nucleic acid purification with this kit?
A: The process involves binding viral nucleic acids to the silica membrane in the supplied spin columns, followed by washing steps to remove impurities and elution of the purified nucleic acids for downstream applications.
Q: Why should I choose this kit for virus detection?
A: The QIAamp UltraSens Virus Kit provides ultrahigh sensitivity and reproducibility, ensuring reliable detection and quantification even of low-titer samples, and is compatible with many laboratory workflows, including QIAcube automation.
Q: What precautions should I observe while using the kit?
A: The kit contains guanidine-based buffers, which are hazardous. Always handle with care, following laboratory safety guidelines and wearing appropriate personal protective equipment.