Qiagen QuantiTect Primer Assay (200)

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Product Details:

Usage Type Laboratory
Shelf Life 12 months (when stored appropriately)
Function Primers for target gene amplification
Instruments Type PCR, Real-Time PCR Instruments
Accuracy High specificity and sensitivity
Features Pre-validated, gene-specific assay; contamination-free; high specificity; optimized for SYBR Green
Storage Instructions Store at 20C
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Qiagen QuantiTect Primer Assay (200) Price And Quantity

Qiagen QuantiTect Primer Assay (200) Product Specifications

Function Primers for target gene amplification
Equipment Type Primer Assay Kit
Technology Real-time PCR, SYBR Green technology
Instruments Type PCR, Real-Time PCR Instruments
Shelf Life 12 months (when stored appropriately)
Usage Type Laboratory
Real-Time Operation Yes
Use Gene expression analysis by real-time RT-PCR and PCR
Condition New
Material Lyophilized, ready-to-use primer mix
Color White (Tube/Container)
Features Pre-validated, gene-specific assay; contamination-free; high specificity; optimized for SYBR Green
Portable Yes
Accuracy High specificity and sensitivity
Wall Mounted No
Operating Type Manual
Storage Instructions Store at 20C

Product Description

Brand Qiagen

Product Type Primer Assay

Pack Size 200 x 50 l

CAT No 249900

Application Molecular Reagent

QuantiTect Primer Assays are genomewide, bioinformatically validated primer sets for use in SYBR Green-based real-time RT-PCR on any cycler. Assays are available for all genes from human, rat, mouse, and many other species. Each assay for a specific gene is supplied as a lyophilized mix of forward and reverse primers that can be easily reconstituted to obtain a 10x assay solution (reaction components for real-time RT-PCR need to be ordered separately). When used in combination with QuantiFast, QuantiTect, Rotor-Gene, or FastLane Kits for SYBR Green detection, QuantiTect Primer Assays guarantee highly specific and sensitive results in real-time RT-PCR that are comparable to probe-based detection.

Reliable Target Amplification for qPCR

With carefully designed, gene-specific primers validated for real-time PCR, the QuantiTect Primer Assay delivers consistent results in gene expression studies. Its optimized formulation eliminates complex preparation steps, ensuring high performance across a range of real-time PCR machines.

Convenient Storage and Simple Reconstitution

The lyophilized primer format offers extended stability-simply store at -20C for up to 12 months. When ready to use, reconstitute the primers with nuclease-free water. This provides researchers flexibility and reduces reagent waste during experimental planning.

Pre-Validated Accuracy and High Sensitivity

Each primer set undergoes stringent quality control to ensure precise and specific amplification, leading to accurate gene quantification in PCR and RT-PCR workflows. The contamination-free tube design also preserves sample integrity.

FAQ's of Qiagen QuantiTect Primer Assay (200):

Q: How do I reconstitute the QuantiTect Primer Assay before use?

A: To prepare the primers for use, simply add nuclease-free water (not included) directly to the lyophilized primers in the single tube. Thorough mixing ensures complete dissolution and readiness for your qPCR or cDNA analysis workflow.

Q: What genes does this kit cover, and can it be used with both human and animal samples?

A: The QuantiTect Primer Assay is designed to target a broad selection of genes from human, mouse, and rat samples, making it suitable for many gene expression studies across these species.

Q: When should I store the product at -20C and how long is it stable?

A: Upon receipt, the assay should be stored at -20C. It remains stable for up to 12 months under these storage conditions, ensuring reliable performance over time.

Q: Where can I use the QuantiTect Primer Assay?

A: The kit is intended for laboratory use, particularly in molecular biology settings equipped for real-time PCR or qPCR research on PCR-compatible instruments.

Q: What is the primary benefit of using this pre-validated primer assay?

A: Utilizing pre-validated primers significantly enhances specificity and sensitivity, reduces the risk of contamination, and saves time compared to designing and optimizing custom primers.

Q: Is special shipping required for this product?

A: No special shipping is required; the kit is shipped at ambient temperature and does not contain hazardous materials, making transport convenient and safe.

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