Product Description
Brand Qiagen
Product Type RNA Kit
Pack Size 250T
CAT No 74636
Application Molecular Reagent
The QIAwave RNA Plus Mini Kit is a more eco-friendly version of our standard RNeasy Plus Mini Kit, combining high-quality RNA purification with effective gDNA removal using unique gDNA Eliminator columns. Total RNA of high purity and yield is obtained from a wide range of cultured cells and easy-to-lyse tissues.
Advanced RNA Purification TechnologyUtilizing optimized silica membrane spin column technology, the QIAwave RNA Plus Mini Kit (250) simplifies RNA isolation, delivering high yields and minimal contamination even from challenging sample types. The process is designed for efficiency and reproducibility, accommodating both novice and experienced researchers.
Broad Sample CompatibilityThis kit efficiently processes up to 30 mg of animal tissue or 10^7 cultured cells or bacteria per preparation, making it versatile for various experimental needs. The components are RNase-free, ensuring integrity of the purified RNA for downstream molecular biology applications.
Eco-Conscious and User-Friendly DesignWith reduced plastic usage and easy-to-follow manual protocols, the QIAwave RNA Plus Mini Kit promotes sustainability and accessibility. Its components are colorless or white, and the kit can be stored at room temperature or partially at 2-8C, supporting lab convenience and workflow flexibility.
FAQ's of Qiagen QIAwave RNA Plus Mini Kit (250):
Q: How do I use the QIAwave RNA Plus Mini Kit (250) for RNA extraction?
A: To isolate RNA, simply process up to 30 mg of animal tissue or 10^7 cells/bacteria per prep using the provided silica-membrane spin columns and proprietary reagents. Follow the manual protocol included to complete RNA purification within approximately 30 minutes.
Q: What types of samples can be processed with this kit?
A: The QIAwave RNA Plus Mini Kit (250) is designed to extract total RNA from a variety of sources, including animal tissues, cultured mammalian cells, and bacteria, accommodating diverse molecular biology research requirements.
Q: When should the kit components be stored at 2-8C?
A: Although most components can be stored at room temperature (15-25C), some specific reagents require storage at 2-8C for optimal stability. Refer to the kit instructions to identify which items need refrigeration.
Q: Where can I use this kit and is it portable?
A: This kit is suitable for laboratory use and is portable, meaning you can easily transport it between different lab environments. However, its use is intended for research only, not for diagnostic or therapeutic purposes.
Q: What is the typical processing time and throughput?
A: The entire RNA extraction and purification process takes approximately 30 minutes per prep, supporting low to medium throughput suitable for routine research applications.
Q: What benefits does this kit offer over traditional RNA extraction methods?
A: The QIAwave RNA Plus Mini Kit delivers high-purity RNA with minimal contamination, offers a fast and efficient workflow, reduces plastic usage, and is compatible with a variety of sample types, all of which streamline routine RNA purification in the lab.