Product Description
Brand Calbiotech
Sample Type Serum
Pack Size 96 Wells
CAT No CP441S
The C-peptide ELISA (enzyme-linked immunosorbent assay) kit is intended for the quantitative determination of human C-peptide levels in human serum.
Purpose and Precision in C-Peptide AssessmentCalbiotech's C-Peptide ELISA kit is expertly developed for researchers and clinicians requiring accurate measurement of human C-Peptide levels. Featuring high sensitivity (0.1 ng/mL) and a broad quantitation range, it supports advanced applications in diabetes and pancreatic studies. The kit's color-coded components, ease of use, and ready-to-use reagents minimize preparation time and maximize experimental reliability. Its manual operation and standardized 96-well format cater efficiently to laboratory workflows.
Optimized Workflow for Reliable ResultsWith an incubation time of around 2 hours and minimal sample volume, the Calbiotech C-Peptide ELISA kit enables rapid and reproducible analysis. The kit includes six calibration standards and controls, ensuring every run achieves robust validation. Results can be read on any standard ELISA plate reader, supporting consistent integration into laboratory processes. Detailed protocols and clear reagent labeling further enhance ease of use, aiding dependable routine testing.
FAQ's of Calbiotech C-Peptide ELISA:
Q: How does the Calbiotech C-Peptide ELISA Kit work for quantitative analysis?
A: This kit employs enzyme-linked immunosorbent assay (ELISA) technology with colorimetric detection. C-Peptide in samples binds to antibodies on the well surface; after adding conjugate and substrate, a color change proportional to C-Peptide concentration occurs, which is measured at a specific wavelength using an ELISA plate reader.
Q: What types of samples and species are compatible with this assay?
A: The Calbiotech C-Peptide ELISA kit is validated exclusively for use with human serum or plasma samples. Its reagents and controls are optimized specifically for human C-Peptide measurement.
Q: When and where is this kit most appropriately used?
A: This ELISA kit is ideal for laboratory settings involved in diabetes research or studies assessing pancreatic function. Its high sensitivity and selectivity make it suitable for clinical and academic laboratories focusing on metabolic or endocrine disorders.
Q: What are the necessary instruments and equipment required for running this kit?
A: A standard laboratory ELISA plate reader is required to read results from the 96-well plate. Other basic lab equipment, such as pipettes and a plate washer (manual or automated), will ensure precise sample and reagent handling.
Q: What is the typical process for performing the C-Peptide ELISA test?
A: First, add 25 L of sample or standard to the wells. After incubation and washing steps, add conjugate and substrate sequentially. Stop the reaction after the final incubation and read the colorimetric output using an ELISA plate reader. The entire process takes about 2 hours.
Q: How should the kit and its reagents be stored for optimal performance?
A: All components should be stored at 2-8C as specified in the kit's storage instructions. Proper storage maintains the shelf life (12 months) and assures reliable assay accuracy (>95%).
Q: What are the main benefits of using this ELISA kit?
A: Key benefits include high assay sensitivity, a broad measurement range, included controls for quality assurance, and ready-to-use reagents. Its streamlined workflow supports efficient, reproducible, and quantitative C-Peptide analysis for biomedical research.