Thermo Fisher Scientific Applied Biosystems TaqMan Universal Master Mix II, with UNG

Thermo Fisher Scientific Applied Biosystems TaqMan Universal Master Mix II, with UNG

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Product Details:

Accuracy High, optimal for quantitative PCR (qPCR)
Measurement Range Dynamic range over 67 orders of magnitude (depending on protocol and target)
Shelf Life 12 months (from date of manufacture)
Features Contains UNG (uracil-N-glycosylase) to prevent carryover contamination, optimized for sensitive and reproducible qPCR results, compatible with TaqMan assays
Storage Instructions Store at -15C to -25C. Do not freeze-thaw repeatedly. Protect from light.
Function Facilitates amplification and detection of DNA targets using TaqMan Probe-Based qPCR
Usage Type Laboratory, Research, Diagnostic
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Thermo Fisher Scientific Applied Biosystems TaqMan Universal Master Mix II, with UNG Price And Quantity

Thermo Fisher Scientific Applied Biosystems TaqMan Universal Master Mix II, with UNG Product Specifications

Technology qPCR (quantitative polymerase chain reaction), TaqMan Probe-Based
Color Clear to slightly yellow liquid
Usage Type Laboratory, Research, Diagnostic
Condition New
Measurement Range Dynamic range over 67 orders of magnitude (depending on protocol and target)
Instruments Type For Applied Biosystems and other compatible real-time PCR instruments
Operating Type Manual/Automatic (for use in compatible qPCR instruments)
Real-Time Operation Yes
Power Source Not required (chemical reagent)
Accuracy High, optimal for quantitative PCR (qPCR)
Material Liquid reagent, proprietary chemical composition
Equipment Type Master Mix Reagent for qPCR
Wall Mounted No
Shelf Life 12 months (from date of manufacture)
Weight Varies by pack size (commonly 1 mL, 5 mL, or larger)
Dimension (L*W*H) Not applicable (liquid reagent packaging)
Portable Yes, in proper transport conditions
Function Facilitates amplification and detection of DNA targets using TaqMan Probe-Based qPCR
Storage Instructions Store at -15C to -25C. Do not freeze-thaw repeatedly. Protect from light.
Features Contains UNG (uracil-N-glycosylase) to prevent carryover contamination, optimized for sensitive and reproducible qPCR results, compatible with TaqMan assays
Use Quantitative real-time PCR, gene expression analysis, pathogen detection

Regulatory Status For Research Use Only. Not for use in diagnostic procedures.
Reaction Setup Premixed, ready-to-use master mix
Enzyme AmpliTaq Gold DNA Polymerase
Mixing Requirement Mix by gentle inversion; do not vortex
Container Type Bottle or tube, size varies (commonly 1 mL or 5 mL)
Compatible Assays All TaqMan Gene Expression, Genotyping, and MicroRNA Assays
Lot Number Tracking Batch-specific quality control and documentation
UNG Component Uracil-N-Glycosylase for prevention of PCR carryover contamination
Buffer Proprietary, includes dNTPs and optimized buffer components
Shipping Conditions Ships on dry ice

Product Description

Brand	Thermo Fisher Scientific
Product Type	Master Mix
Pack Size	10 x 5 mL
CAT No	4440046
Application	Molecular Biology

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.

Reliable qPCR Performance

This master mix is optimized for high accuracy and sensitive quantification in qPCR applications, ensuring reliable results even at low target concentrations. The inclusion of carefully balanced buffer components and AmpliTaq Gold DNA Polymerase leads to consistent amplification, enabling users to trust their gene expression, genotyping, or microRNA analysis outcomes.

Carryover Contamination Prevention

The incorporation of Uracil-N-Glycosylase (UNG) significantly reduces the risk of PCR carryover contamination by degrading any uracil-containing DNA from previous PCR reactions. This feature is crucial for preventing false positives in sensitive qPCR experiments, especially in high-throughput laboratory environments with repeated sample handling.

Ready-to-Use Convenience

Delivered as a premixed liquid reagent, this master mix reduces preparation time and user error. Simply mix by gentle inversion and it's ready for immediate use. The master mix is compatible with both manual and automated pipetting systems on a wide range of real-time PCR instruments, which makes it ideal for various laboratory workflows.

Storage and Stability

To maintain its stability and performance, the master mix should be stored at -15C to -25C, protected from light, and avoid repeated freeze-thaw cycles. It is shipped on dry ice for optimal preservation, with a shelf life of 12 months from the date of manufacture, supporting extended use in research laboratories.

FAQ's of Thermo Fisher Scientific Applied Biosystems TaqMan Universal Master Mix II, with UNG:

Q: How do I properly mix the TaqMan Universal Master Mix II with UNG before use?

A: Gently invert the master mix several times to ensure homogeneity. Do not vortex the solution, as vigorous mixing can introduce bubbles and negatively affect the performance in qPCR assays.

Q: What types of assays are compatible with this master mix?

A: The mix is compatible with all TaqMan Gene Expression, Genotyping, and MicroRNA Assays, making it suitable for a wide range of qPCR applications, including gene expression studies and pathogen detection.

Q: When and why should I use a master mix containing UNG?

A: UNG (Uracil-N-Glycosylase) is included to prevent PCR carryover contamination by degrading uracil-containing DNA from previous experiments. It is especially beneficial in environments where cross-contamination risk is a concern or when working with high-sensitivity qPCR assays.

Q: Where should I store the TaqMan Universal Master Mix II with UNG, and how should it be handled upon arrival?

A: Store the master mix at -15C to -25C immediately upon arrival. Protect it from light, and avoid repeated freeze-thaw cycles to ensure product integrity and consistent qPCR results.

Q: What are the benefits of using a premixed, ready-to-use master mix?

A: Using a premixed, ready-to-use master mix reduces preparation time, minimizes pipetting errors, and ensures consistent component concentrations for reproducible qPCR results. It simplifies the workflow and increases laboratory efficiency.

Q: How is the product quality monitored and tracked?

A: Each lot of the master mix undergoes stringent batch-specific quality control and documentation, ensuring reliable performance and traceability for every batch manufactured.

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