Qiagen dPCR Microbial DNA Detection Assays

Qiagen dPCR Microbial DNA Detection Assays

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Product Details:

Accuracy High, dependent on dPCR instrument
Usage Type Laboratory
Storage Instructions Store at -20C, avoid repeated freeze-thaw cycles
Function Detection and quantification of microbial DNA
Shelf Life 12 months
Instruments Type Requires compatible digital PCR (dPCR) system
Features High sensitivity and specificity, ready-to-use assays, multiplex detection
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Qiagen dPCR Microbial DNA Detection Assays Price And Quantity

Qiagen dPCR Microbial DNA Detection Assays Product Specifications

Storage Instructions Store at -20C, avoid repeated freeze-thaw cycles
Usage Type Laboratory
Technology Digital PCR (dPCR)
Condition New
Color Standard lab kit colors (may vary)
Function Detection and quantification of microbial DNA
Wall Mounted No
Power Source Compatible with real-time dPCR instruments (instrument-dependent)
Power Consumption Instrument dependent
Accuracy High, dependent on dPCR instrument
Operating Type Automatic
Real-Time Operation Yes
Material Specialized reagents and oligonucleotides
Weight Box/kit size varies
Shelf Life 12 months
Use Microbial DNA detection and quantification
Instruments Type Requires compatible digital PCR (dPCR) system
Equipment Type dPCR Microbial DNA Detection Assays
Portable No
Measurement Range Dynamic range as per dPCR system specifications
Dimension (L*W*H) Box/kit size varies
Features High sensitivity and specificity, ready-to-use assays, multiplex detection
Voltage Instrument dependent

Product Description

Brand Qiagen

Product Type dPCR Kit

Pack Size 200 x 40 L

CAT No 250207

Application Molecular Reagent

Microbial identification and profiling are of interest to many fields including human health, food and feed testing and environmental testing. Microbial identification determines the presence or absence of a microbe in a sample, while microbial profiling determines its relative expression under two or more experimental conditions, thus requiring a reference sample and a normalizer.

High-Sensitivity Microbial DNA Detection

Leverage the accuracy and sensitivity of digital PCR technology to detect and quantify microbial DNA from bacteria, viruses, and fungi. With pre-validated primer/probe mixes and optimized assay components, these kits deliver reliable results for both single and multiplex detection, making them suitable for a variety of research and diagnostic applications.

Streamlined Workflow for High Throughput

The ready-to-use format enables rapid assay setup, minimizing preparation time while supporting high-throughput operations in 96- or 384-well plate formats. Each assay kit contains the necessary reagents and controls, allowing seamless integration with compatible dPCR instruments and supporting a wide range of laboratory demands.

Reliable Components and Comprehensive Support

Each kit includes proprietary reagents, DNAs, and positive/negative controls for consistent performance. The shelf life is 12 months and kits should be stored at -20C to ensure stability. Qiagen provides detailed protocols and responsive technical support, helping users maximize assay performance and data quality.

FAQ's of Qiagen dPCR Microbial DNA Detection Assays:

Q: How do I use the Qiagen dPCR Microbial DNA Detection Assays in my laboratory workflow?

A: To use the Qiagen dPCR Microbial DNA Detection Assays, simply prepare your DNA sample, add it to the provided master mix with the pre-validated primer/probe mix, and follow the step-by-step protocol included in the kit. Load the reactions into a compatible digital PCR instrument and initiate the automatic run for microbial DNA detection and quantification.

Q: What sample types are suitable for these dPCR assays?

A: These assays are optimized for DNA extracted from diverse microbial samples, including bacteria, viruses, and fungi. This flexibility allows detection and quantification from clinical, environmental, or food safety testing materials.

Q: When should I choose single or multiplex detection formats?

A: Select the single detection format when targeting one microbial species per assay, while multiplex detection is ideal for analyzing multiple microbial targets simultaneously in the same run. The choice depends on your research or testing objectives and sample complexity.

Q: Where can I use these assays, and are they compatible with non-Qiagen instruments?

A: Qiagen dPCR Microbial DNA Detection Assays are designed for laboratory use and are compatible with Qiagen as well as other leading digital PCR platforms. Check your instrument's specifications to ensure compatibility with 96- or 384-well formats.

Q: What is included in each assay kit, and how is it stored?

A: Each kit contains a proprietary primer/probe mix, DNA standards, positive and negative controls, and a dPCR master mix. For optimal performance, store all components at -20C and avoid repeated freeze-thaw cycles.

Q: What are the major benefits of using these Qiagen dPCR assays?

A: Major advantages include high sensitivity and specificity, ready-to-use convenience, robust multiplex detection, and consistent, reproducible results. The assays also support high-throughput capabilities and come with comprehensive user guidance.

Q: What technical support is available if I have questions during the process?

A: Qiagen offers detailed protocols with each kit as well as professional technical support. This ensures any difficulties or questions you encounter while using the assays are promptly addressed by knowledgeable specialists.

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