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Qiagen QIAcuity OneStep Advanced Probe Kit (1 ml) Qiagen QIAcuity OneStep Advanced Probe Kit (1 ml) Qiagen QIAcuity OneStep Advanced Probe Kit (1 ml)
Qiagen QIAcuity OneStep Advanced Probe Kit (1 ml)
Qiagen QIAcuity OneStep Advanced Probe Kit (1 ml) Qiagen QIAcuity OneStep Advanced Probe Kit (1 ml)

Qiagen QIAcuity OneStep Advanced Probe Kit (1 ml)

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Product Details:

  • Usage Type For laboratory and research use only
  • Storage Instructions Store at -15C to -25C
  • Shelf Life 12 months (when stored as recommended)
  • Function Amplifies and quantifies specific DNA/RNA sequences in digital PCR
  • Features One-step reaction, high specificity, optimized for probe-based dPCR, advanced sensitivity
  • Measurement Range Broad dynamic quantification range for DNA and RNA targets
  • Instruments Type For use with QIAcuity Digital PCR Instruments
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Qiagen QIAcuity OneStep Advanced Probe Kit (1 ml) Price And Quantity

Qiagen QIAcuity OneStep Advanced Probe Kit (1 ml) Product Specifications

  • Approximately 1 ml reagent kit
  • Not Applicable (reagent kit packaging)
  • Automated or manual dPCR workflows
  • Probe-based quantification and detection of nucleic acids by dPCR
  • No
  • Silent operation
  • Amplifies and quantifies specific DNA/RNA sequences in digital PCR
  • One-step reaction, high specificity, optimized for probe-based dPCR, advanced sensitivity
  • For use with QIAcuity Digital PCR Instruments
  • Digital PCR (dPCR)
  • High, suitable for precise nucleic acid quantification
  • OneStep Advanced Probe Kit
  • Store at -15C to -25C
  • Colorless to slightly yellow (reagents)
  • Broad dynamic quantification range for DNA and RNA targets
  • 12 months (when stored as recommended)
  • New
  • For laboratory and research use only
  • PCR reagent chemicals
  • Yes, compatible with real-time detection
  • Yes (kit format)

Product Description

Brand Qiagen

Product Type RT PCR Kit

Pack Size 1 ml

CAT No 250131

Application Molecular Reagent



The QIAcuity OneStep Advanced Probe PCR Kit enables sensitive quantification of RNA or RNA+DNA targets on the QIAcuity digital PCR instrument in one combined RT-PCR reaction. It contains a 4x concentrated Master Mix and a 100x concentrated RT Mix optimized for microfluidic use in the QIAcuity Nanoplates. The kit enhances the specificity and efficiency of probe-based digital PCR to provide accurate, singleplex or up to 5-plex analysis.




Seamless dPCR Workflow Integration

The QIAcuity OneStep Advanced Probe Kit integrates smoothly with QIAcuity Digital PCR Instruments, enabling researchers to confidently execute probe-based nucleic acid quantification. Its ready-to-use format reduces setup time while maintaining accuracy throughout the workflow.


High Sensitivity and Specificity for Critical Applications

This kit features an advanced one-step RT-PCR master mix optimized for multiplexing and precise detection of low-abundance targets. Its robust formulation supports a broad quantitative range, making it ideal for absolute quantification, rare mutation analysis, and gene expression profiling.


Stability and Performance You Can Trust

Packaged with advanced stabilization reagents and a tamper-evident cap, the kit assures reagent integrity for up to 12 months when stored at -15C to -25C. Each lot is traceable via number, ensuring high reliability batch after batch.

FAQ's of Qiagen QIAcuity OneStep Advanced Probe Kit (1 ml):


Q: How do I use the QIAcuity OneStep Advanced Probe Kit in my dPCR workflow?

A: Simply combine your sample with the kit components as outlined in the provided protocol and load them onto the QIAcuity dPCR System. The kit supports both single-step RT-PCR and probe-based digital PCR for straightforward and efficient workflows.

Q: What applications is this kit recommended for?

A: The kit is recommended for absolute quantification, rare mutation detection, and gene expression analysis of DNA and RNA using probe-based digital PCR assays.

Q: When should I use this kit over traditional PCR kits?

A: Use this kit when you require high sensitivity, precise quantification, or need to detect rare events, as it is specifically formulated for digital PCR applications, offering better accuracy and dynamic range than many traditional PCR kits.

Q: Where can I find the lot number and regulatory status of my kit?

A: The lot number and regulatory status are provided directly on the product packaging. This ensures traceability and compliance with research use regulations.

Q: What are the storage instructions for the kit to maintain its shelf life?

A: Store the Qiagen QIAcuity OneStep Advanced Probe Kit at -15C to -25C immediately upon receipt to maintain reagent stability and ensure up to 12 months of reliable performance.

Q: How does the kit benefit my digital PCR assays?

A: The kit delivers high specificity, advanced sensitivity, and compatibility with multiplex and probe-based workflows, making it ideal for demanding dPCR applications involving DNA and RNA quantification.

Q: Can the kit be used for diagnostic or clinical purposes?

A: No, this product is intended for research use only and is not suitable for clinical or diagnostic procedures.

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