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Thermo Fisher Scientific Invitrogen SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase Thermo Fisher Scientific Invitrogen SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase Thermo Fisher Scientific Invitrogen SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase Thermo Fisher Scientific Invitrogen SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase Thermo Fisher Scientific Invitrogen SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase
Thermo Fisher Scientific Invitrogen SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase
Thermo Fisher Scientific Invitrogen SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase Thermo Fisher Scientific Invitrogen SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase Thermo Fisher Scientific Invitrogen SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase Thermo Fisher Scientific Invitrogen SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase

Thermo Fisher Scientific Invitrogen SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase

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Product Details:

  • Storage Instructions Store at -20C
  • Measurement Range Low femtogram to microgram amounts of RNA
  • Instruments Type Molecular biology reagent kit
  • Function cDNA synthesis and PCR amplification from RNA samples
  • Features One-tube format, minimized contamination risk, high sensitivity and specificity, includes SuperScript III RT and Platinum Taq DNA Polymerase
  • Shelf Life 12 months (when properly stored)
  • Usage Type Laboratory research
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Thermo Fisher Scientific Invitrogen SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase Price And Quantity

Thermo Fisher Scientific Invitrogen SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase Product Specifications

  • Enzyme-based, reagents in buffered solution
  • No
  • One-Step RT-PCR with Platinum Taq DNA Polymerase
  • Low femtogram to microgram amounts of RNA
  • Reverse transcription and amplification of RNA targets in a single step
  • 12 months (when properly stored)
  • Clear/Colorless reagents
  • Laboratory research
  • High specificity and sensitivity for RNA targets
  • RT-PCR System
  • Not Applicable (used with compatible thermal cycler)
  • Automated Thermal Cycler required
  • No, endpoint RT-PCR
  • Molecular biology reagent kit
  • Yes (reagent kit)
  • cDNA synthesis and PCR amplification from RNA samples
  • New
  • Not Applicable (kit form)
  • One-tube format, minimized contamination risk, high sensitivity and specificity, includes SuperScript III RT and Platinum Taq DNA Polymerase
  • Store at -20C

Product Description

Brand Thermo Fisher Scientific
Product Type RT-PCR Kit
Pack Size 100 Reactions
CAT No 10928042
Application Molecular Biology

SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR. This system combines RT/Platinum Taq mix and 2X reaction mix. The RT/Platinum Taq mix contains a mixture of SuperScript II Reverse Transcriptase and Platinum Taq DNA Polymerase. The 2X reaction mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg2+ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers.



Optimized One-Step RT-PCR Workflow

The Invitrogen SuperScript One-Step RT-PCR System is designed for efficient and accurate conversion of RNA to cDNA followed by PCR amplification in a single streamlined protocol. By integrating reverse transcriptase and hot-start Taq polymerase in a one-tube format, the system minimizes pipetting steps, lowers the risk of contamination, and saves valuable laboratory time. The provided proprietary buffer further enhances reaction efficiency and specificity, catering to applications requiring high sensitivity.


Flexible Kit Sizes and Broad Sample Compatibility

This system is available in kit sizes accommodating 25, 100, or 500 reactions, making it suitable for various project scales. It effectively amplifies targets from total RNA, poly(A)+ RNA, or in vitro transcribed RNA. With the capability to process samples ranging from femtograms to micrograms, it supports a wide spectrum of experimental requirements across gene expression research, viral detection, and molecular cloning.


Hot-Start PCR and Superior Enzyme Performance

Incorporating Platinum Taq DNA Polymerase with antibody-mediated inhibition grants a hot-start capability, improving specificity by preventing non-specific amplification at lower temperatures. SuperScript III Reverse Transcriptase features minimal RNase H activity, ensuring efficient synthesis of full-length cDNA even for amplicons up to 4.5 kb. The included RNase inhibitor further safeguards RNA integrity during the reaction, contributing to high accuracy and reproducibility.

FAQ's of Thermo Fisher Scientific Invitrogen SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase:


Q: How does the SuperScript One-Step RT-PCR System minimize contamination risk during RNA amplification?

A: The system utilizes a one-tube format, allowing both reverse transcription and PCR amplification to be conducted without transferring samples between tubes. This design reduces pipetting steps and significantly lowers the risk of cross-contamination.

Q: What types of RNA samples are compatible with this kit?

A: The kit can be used with total RNA, poly(A)+ RNA, or in vitro transcribed RNA, offering flexibility for diverse molecular biology applications.

Q: When should I use this kit versus separate RT and PCR reactions?

A: This kit is ideal when you require fast, streamlined processing and wish to minimize handling errors or contamination. It is suitable for gene expression analysis, pathogen detection, and other applications requiring sensitive RNA amplification in a single step.

Q: Where should the kit be stored, and what is the recommended storage temperature?

A: For optimal shelf life, store the kit at -20C. It can be kept at 4C for up to 24 hours during short-term use. Always follow the manufacturer's guidelines to maintain reagent integrity.

Q: What is the maximum amplicon size that can be achieved with this system?

A: The system supports amplification of RNA targets up to 4.5 kilobases, making it suitable for a wide range of gene targets.

Q: How long does a typical reaction take using this kit?

A: A complete RT-PCR reaction, including both cDNA synthesis and PCR amplification, generally takes about 80 minutes when following the recommended protocol.

Q: What are the key benefits of using Platinum Taq DNA Polymerase with hot-start capability in this kit?

A: The hot-start mechanism, achieved through antibody inhibition, increases PCR specificity by preventing non-specific amplification and primer-dimer formation during reaction setup, resulting in cleaner and more reliable results.

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