Thermo Fisher Scientific Invitrogen Platinum SuperFi II DNA Polymerase

Thermo Fisher Scientific Invitrogen Platinum SuperFi II DNA Polymerase

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Product Details:

Accuracy >300X higher fidelity than Taq DNA Polymerase
Function DNA synthesis during PCR amplification
Shelf Life 12 months (when stored properly)
Measurement Range Up to 10 kb (for PCR products)
Instruments Type PCR Reagent
Features High specificity, high fidelity, fast PCR, optimized for difficult templates
Storage Instructions Store at -20 C
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Thermo Fisher Scientific Invitrogen Platinum SuperFi II DNA Polymerase Price And Quantity

Buffer System Included, optimized for Platinum SuperFi II
Hot Start Technology YesPlatinum Antibody-mediated Hot Start
Sample Throughput Low to High (Scalable for different PCR volumes)
Application Routine and complex PCR, cloning, mutagenesis, genotyping
Product Code/Part Number 12359010, 12359011 (examples)
Template Compatibility Genomic DNA, cDNA, plasmid DNA, GC-rich or challenging templates
Shipping Temperature On dry ice or cold packs
Compatibility Compatible with 96-well and 384-well PCR platforms
Enzyme Concentration 2 units/L
Instruction Manual Provided with product
Formulation 2X Master Mix and Standalone Enzyme
Package Size 100 reactions, 500 reactions, varied kits
Expiration Date Printed on packaging

Thermo Fisher Scientific Invitrogen Platinum SuperFi II DNA Polymerase Product Specifications

Accuracy >300X higher fidelity than Taq DNA Polymerase
Measurement Range Up to 10 kb (for PCR products)
Instruments Type PCR Reagent
Condition New
Color Clear or Colourless Solution
Technology High-Fidelity PCR Enzyme
Material Enzyme (Recombinant Protein), Buffer Components
Real-Time Operation Compatible with Real-Time PCR
Features High specificity, high fidelity, fast PCR, optimized for difficult templates
Portable Yes
Usage Type Laboratory/Reagent
Shelf Life 12 months (when stored properly)
Use PCR Amplification, DNA Analysis
Weight Generally <1 kg (per kit)
Function DNA synthesis during PCR amplification
Storage Instructions Store at -20 C
Dimension (L*W*H) Product Vials (Standard Packaging)
Wall Mounted No
Operating Type Manual/Pipetting
Equipment Type DNA Polymerase

Buffer System Included, optimized for Platinum SuperFi II
Hot Start Technology YesPlatinum Antibody-mediated Hot Start
Sample Throughput Low to High (Scalable for different PCR volumes)
Application Routine and complex PCR, cloning, mutagenesis, genotyping
Product Code/Part Number 12359010, 12359011 (examples)
Template Compatibility Genomic DNA, cDNA, plasmid DNA, GC-rich or challenging templates
Shipping Temperature On dry ice or cold packs
Compatibility Compatible with 96-well and 384-well PCR platforms
Enzyme Concentration 2 units/L
Instruction Manual Provided with product
Formulation 2X Master Mix and Standalone Enzyme
Package Size 100 reactions, 500 reactions, varied kits
Expiration Date Printed on packaging

Product Description

Brand	Thermo Fisher Scientific
Product Type	DNA Polymerase
Pack Size	5 x 500 Reactions
CAT No	12361250
Application	Molecular Biology

Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR. Due to the unique composition of the SuperFi II PCR buffer, the annealing temperature is 60AC for most primer pairs designed following the general design rules.

Precision and Reliability for Every PCR

Platinum SuperFi II DNA Polymerase guarantees high-fidelity DNA amplification using advanced Platinum antibody-mediated hot start technology. Its robust performance ensures accurate results for genotyping, cloning, mutagenesis, and more, even with complex or GC-rich templates. Integrated compatibility with standard laboratory PCR hardware means seamless adaptation across diverse workflows.

Flexible Solutions for Every Laboratory Need

This DNA polymerase comes packaged in 100-reaction and 500-reaction kits and offers both a convenient 2X Master Mix and standalone enzyme format. Designed for flexibility, Platinum SuperFi II can accommodate low to high sample throughputs, supporting both routine and advanced research projects in scalable volumes. Whether you're working in a small lab or a high-throughput environment, this reagent fits your process.

User-Friendly and Ready for Rigorous Demands

Shipping on dry ice or cold packs and requiring storage at -20C, this solution ensures long-term viability with a shelf life of 12 months. An included instruction manual and a clear, colorless formulation make handling and pipetting straightforward. The Enzyme's high specificity and exceptional fidelity enhance confidence in every result, making it ideal for demanding applications.

FAQ's of Thermo Fisher Scientific Invitrogen Platinum SuperFi II DNA Polymerase:

Q: How do I use Invitrogen Platinum SuperFi II DNA Polymerase in my PCR workflow?

A: To use this polymerase, simply follow the protocol in the provided instruction manual, combining either the 2X Master Mix or standalone enzyme with your DNA template, primers, and nuclease-free water. The enzyme works with a variety of PCR platforms in both 96-well and 384-well formats, ensuring broad compatibility.

Q: What types of templates can be amplified with this enzyme?

A: Platinum SuperFi II DNA Polymerase is optimized for a wide range of templates including genomic DNA, cDNA, plasmid DNA, as well as GC-rich and other challenging sequences. This versatility makes it suitable for both routine and complex PCR applications.

Q: When should I choose the 2X Master Mix versus the standalone enzyme format?

A: The 2X Master Mix is ideal for routine and high-throughput workflows, simplifying reaction setup and minimizing errors. The standalone enzyme offers greater flexibility, enabling users to customize buffer and reaction conditions for specialized applications.

Q: Where should I store the polymerase, and how long does it last?

A: Store Platinum SuperFi II DNA Polymerase at -20C for optimal stability. When stored properly, the enzyme maintains performance for up to 12 months, with the expiration date clearly printed on the product packaging.

Q: What are the benefits of Platinum antibody-mediated hot start technology?

A: The hot start feature prevents non-specific amplification and primer-dimer formation by keeping the enzyme inactive until the reaction is heated. This ensures higher specificity and clean PCR results, especially beneficial in complex or low-template samples.

Q: How does Platinum SuperFi II DNA Polymerase improve PCR accuracy and efficiency?

A: With over 300X higher fidelity compared to standard Taq DNA Polymerase, Platinum SuperFi II offers exceptional accuracy for cloning, mutagenesis, and DNA analysis. Its optimized buffer and rapid cycling capabilities enable efficient and reproducible amplification of even difficult templates.

Q: Is this enzyme suitable for real-time PCR and what is its measurement range?

A: Yes, Platinum SuperFi II DNA Polymerase is compatible with real-time PCR applications and can reliably amplify DNA fragments up to 10 kb in length, making it suitable for a wide range of analytical and preparative PCR needs.

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