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Qiagen dPCR LNA Mutation Assay (200) Qiagen dPCR LNA Mutation Assay (200) Qiagen dPCR LNA Mutation Assay (200)
Qiagen dPCR LNA Mutation Assay (200)
Qiagen dPCR LNA Mutation Assay (200) Qiagen dPCR LNA Mutation Assay (200)

Qiagen dPCR LNA Mutation Assay (200)

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Product Details:

  • Features High sensitivity and specificity; LNA for improved mismatch discrimination; Ready to use; Universal protocol compatible with multiple dPCR platforms
  • Function Detection and quantification of specific mutations in genomic DNA
  • Accuracy Single-molecule detection, highly accurate mutant allele quantification
  • Storage Instructions Store at -20C; avoid repeated freeze-thaw cycles
  • Shelf Life 12 months (when stored as recommended)
  • Instruments Type Digital PCR instrument (compatible with QIAcuity or equivalent systems)
  • Usage Type Laboratory
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Qiagen dPCR LNA Mutation Assay (200) Price And Quantity

Qiagen dPCR LNA Mutation Assay (200) Product Specifications

  • Colorless or as indicated by assay
  • Proprietary reagent mixes with LNA (Locked Nucleic Acid) technology
  • Detection and quantification of specific mutations in genomic DNA
  • No
  • 12 months (when stored as recommended)
  • Yes (kit format, easily transportable)
  • Digital PCR (dPCR) with LNA probes
  • Not Applicable (consumable assay kit)
  • New
  • dPCR LNA Mutation Assay Kit
  • Store at -20C; avoid repeated freeze-thaw cycles
  • Automated/Manual (depending on dPCR system)
  • Single-molecule detection, highly accurate mutant allele quantification
  • Yes
  • High sensitivity and specificity; LNA for improved mismatch discrimination; Ready to use; Universal protocol compatible with multiple dPCR platforms
  • Not Applicable (reagents for use with dPCR systems)
  • Detection and quantification of somatic mutations
  • 0.1% 100% mutant allele frequency (MAF)
  • Laboratory
  • Digital PCR instrument (compatible with QIAcuity or equivalent systems)

Product Description

Brand                            Qiagen

Product Type               dPCR Kit

Pack Size                     200 x 40 L

CAT No                         250200

Application                  Molecular Reagent



dPCR LNA Mutation Assays enable detection of individual sequence mutations selected from comprehensive curated databases such as COSMIC. The assay designs have been dPCR wet-lab tested, and the resulting data is available. The sensitivity for detecting the target mutation is 0.1% in a wild-type background in one well of aQIAcuity Nanoplate. Even higher sensitivity can be achieved by splitting the reaction into multiple wells and combining the analysis. The choice of two different fluorescent dye combinations allows detection of mutant and wild-type sequences as well as multiplexing analysis of two target mutations in one well.




Unmatched Sensitivity and Specificity

With its advanced LNA probe chemistry, the Qiagen dPCR LNA Mutation Assay achieves extremely high sensitivity and specificity, ensuring reliable detection of rare somatic mutations down to 0.1% mutant allele frequency. Its innovative design minimizes the risk of false positives, providing greater confidence in your mutation quantification results.


Versatile Compatibility and Universal Protocols

This kit is compatible with both the QIAcuity Digital PCR System and a range of third-party dPCR instruments. Its universal protocol streamlines workflows, allowing seamless integration into most laboratory setups. The assay mix is ready-to-use, making it easy to implement for diverse genomic research and clinical applications.


Streamlined Workflow and Flexible Applications

Each kit contains sufficient reagents for 200 reactions, supporting high-throughput projects. The kit accommodates multiple DNA sample types and is suitable for both automated and manual operation, depending on the dPCR system in use. With clear storage instructions and a long shelf life, it is a reliable asset for mutation detection studies.

FAQ's of Qiagen dPCR LNA Mutation Assay (200):


Q: How does the Qiagen dPCR LNA Mutation Assay improve detection of low-frequency somatic mutations?

A: The assay uses Locked Nucleic Acid (LNA) hydrolysis probes, which enhance mismatch discrimination and enable highly sensitive detection of rare somatic mutations, detecting mutant alleles at frequencies as low as 0.1%.

Q: What sample types can be used with this mutation assay kit?

A: You can use genomic DNA, cell-free DNA, or FFPE tissue DNA with this kit, enabling flexibility across different sample sources for research or clinical applications.

Q: When should I use a no template or positive control in my assay?

A: It is recommended to include a no template control to monitor contamination and a positive control to confirm assay functionality with each experiment. However, these controls are not included in the kit and must be sourced separately.

Q: Where can the Qiagen dPCR LNA Mutation Assay be used?

A: This kit is designed for laboratory use and is ideal for settings involved in molecular diagnostics, genetic research, or mutation screening using digital PCR technology.

Q: What is the process for running a reaction with this kit?

A: You add the assay mix, your DNA sample, and PCR master mix (if not provided in the kit) into reaction wells, following the universal protocol and running the assay on a compatible dPCR instrument such as the QIAcuity System.

Q: How does the kit benefit mutation analysis workflows?

A: The kit provides highly accurate quantification of specific mutations at single-molecule sensitivity, features a ready-to-use format, universal compatibility, and supports streamlined, reliable workflows for high-throughput analysis.

Q: What are the storage requirements and shelf life for the reagents?

A: Store the kit at -20C and avoid repeated freeze-thaw cycles. When stored as recommended, the reagents remain stable for up to 12 months.

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