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Thermo Fisher Scientific Invitrogen SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase
Product Details:
- Instruments Type Molecular Biology Reagents
- Usage Type Laboratory, molecular biology research
- Shelf Life 12 months
- Features High sensitivity, robust performance, efficient amplification
- Storage Instructions Store at -20C
- Accuracy High Fidelity for cDNA synthesis and PCR
- Function Amplifies RNA templates via reverse transcription and PCR
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Thermo Fisher Scientific Invitrogen SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase Price And Quantity
Thermo Fisher Scientific Invitrogen SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase Product Specifications
- Yes
- Molecular Biology Reagents
- Compatible with real-time PCR
- No
- New
- Store at -20C
- High sensitivity, robust performance, efficient amplification
- Laboratory, molecular biology research
- 12 months
- One-Step RT-PCR System
- Manual and Automated
- Amplifies RNA templates via reverse transcription and PCR
- Multi-color reagents
- Reagent kit
- cDNA synthesis and PCR amplification from RNA
- Reverse Transcription Polymerase Chain Reaction (RT-PCR)
- High Fidelity for cDNA synthesis and PCR
Product Description
| Brand | Thermo Fisher Scientific |
| Product Type | PCR Amplified cDNA |
| Pack Size | 100T |
| CAT No | 12574018 |
| Application | Molecular Biology |
The SuperScript III One-Step RT-PCR System with PlatinumTaqDNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR. Using this convenient one-step formulation, you can perform both cDNA synthesis and PCR amplification in a single tube using gene-specific primers and target RNAs from either total RNA or mRNA. The system uses a mixture of SuperScript III Reverse Transcriptase and PlatinumTaqDNA polymerase in an optimized reaction buffer, and it can detect a wide range of RNA targets, from 200 bp to 4.5 kb.
Versatile One-Step RT-PCR Solution
Combining reverse transcription and PCR in a single tube, the Invitrogen SuperScript III One-Step RT-PCR System streamlines your workflow while ensuring efficient amplification. Suitable for multiple RNA typesincluding total RNA, poly(A)+ RNA, viral RNA, and in vitro transcribed RNAthe system delivers results with high sensitivity across various sample sources.
High Performance and Reliability
Engineered for robust performance, this reagent kit features SuperScript III Reverse Transcriptase for accurate cDNA synthesis and Platinum Taq DNA Polymerase for efficient PCR. The high-fidelity reaction ensures precision even with longer targets up to 4.5 kb, making it suitable for demanding molecular biology applications.
Flexible and Compatible Design
Offered in convenient pack sizes, this system fits both manual and automated setups. Its compatibility with standard and real-time PCR instruments provides flexibility for researchers, while multi-color reagents enhance usability. Control RNA templates ensure reliable experiment validation, supporting a range of laboratory needs.
FAQs of Thermo Fisher Scientific Invitrogen SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase:
Q: How does the SuperScript III One-Step RT-PCR System simplify cDNA synthesis and PCR amplification?
A: This system combines reverse transcription and PCR steps within a single tube, allowing users to synthesize cDNA from RNA and amplify it by PCR in one streamlined process, reducing hands-on time and the risk of contamination.Q: What types of RNA samples can I use with this RT-PCR kit?
A: You can use total RNA, poly(A)+ RNA, viral RNA, or in vitro transcribed RNA with this system. Its robust formulation allows for high sensitivity and efficient performance across various sample types.Q: When should I use the SuperScript III One-Step RT-PCR System in my research?
A: This system is ideal when you need rapid and reliable conversion of RNA to cDNA followed by amplificationtypically in gene expression studies, viral detection, or molecular cloning applications where efficiency and accuracy are critical.Q: Where can this kit be used, and which equipment is it compatible with?
A: Designed for laboratory and molecular biology research, the kit can be used with both standard and real-time PCR instruments. It supports both manual and automated workflows, providing flexibility for diverse research environments.Q: What is the process for setting up a reaction with this kit?
A: Simply combine your RNA sample with the provided 2X Reaction Mix, Enzyme Mix, and (optionally) the control RNA template in one tube. The reaction is then run on a compatible PCR instrument following the recommended protocol included in the kit.Q: How should I store the reagents, and what is the shelf life?
A: All components should be stored at -20C and remain stable for up to 12 months from the date of receipt, ensuring reliable results throughout this period.Q: What are the main benefits of using this one-step RT-PCR system?
A: The main advantages include high sensitivity, robust amplification up to 4.5 kb targets, reduced hands-on time, minimized contamination risk, compatibility with various instruments, and high-fidelity results suitable for molecular biology research.Tell us about your requirement
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