Qiagen miRCURY LNA miRNA PCR Assay

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Product Details:

Accuracy High detection specificity for miRNAs
Instruments Type PCR Assay Kit
Usage Type Laboratory
Features Ultra-sensitive, high specificity, and simple workflow
Storage Instructions Store at -20C
Measurement Range Low to high abundance miRNAs
Shelf Life As indicated on package (typically 12-24 months)
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Qiagen miRCURY LNA miRNA PCR Assay Price And Quantity

Qiagen miRCURY LNA miRNA PCR Assay Product Specifications

Equipment Type miRNA PCR Assay Kit
Technology LNA-enhanced microRNA PCR (Locked Nucleic Acid)
Power Source Not Required (consumable assay)
Condition New
Usage Type Laboratory
Operating Type Manual
Features Ultra-sensitive, high specificity, and simple workflow
Color Clear / Colorless reagents
Function Quantitative PCR-based detection
Use Detection and quantification of microRNAs
Accuracy High detection specificity for miRNAs
Real-Time Operation Yes
Measurement Range Low to high abundance miRNAs
Material Reagents and consumables for PCR
Storage Instructions Store at -20C
Wall Mounted No
Portable Yes
Dimension (L*W*H) Not Applicable (consumable product)
Instruments Type PCR Assay Kit
Weight Not Applicable (consumable product)
Shelf Life As indicated on package (typically 12-24 months)

Data Output CT values for relative quantification
Assay Format Individual assays or panels
Sensitivity Detects less than 1 pg total RNA input
Compatible Equipment Real-time PCR systems (e.g., Roche LightCycler, Applied Biosystems, Bio-Rad)
Application Gene expression analysis, biomarker discovery, validation
Sample Type Serum, plasma, tissue, cells, urine, and other biological materials
Target MicroRNAs (miRNAs)
Enzyme Included Reverse transcriptase and polymerase supplied
Number of Assays Choose from 12, 24, 60, or 96 well formats
Primer Design Pre-designed, sequence-specific LNA primers included

Product Description

Brand Qiagen

Product Type miRNA Kit

Pack Size 1 pg total RNA

CAT No 339306

Application Molecular Reagent

miRCURY LNA miRNA PCR Assays are individual miRNA PCR primer sets that enable extremely sensitive and specific miRNA quantification with themiRCURY LNA miRNA PCR System. Both forward and reverse PCR amplification primers are miRNA-specific and are optimized withLNA technology. Assays are available in tube or plate format with 200 reactions per tube or well.

Advanced LNA-Enhanced Detection

Benefit from Locked Nucleic Acid (LNA)-enhanced technology which provides heightened specificity and sensitivity, facilitating detection of even low-abundance miRNAs. The pre-designed primers ensure that your assays target precisely the microRNAs of interest, minimizing false positives and enhancing the reliability of your gene expression analyses.

Versatile Sample Compatibility

This PCR assay accommodates a broad spectrum of samples, including serum, plasma, tissue, cells, urine, and more. Its flexibility enables researchers to work with various biological materials for comprehensive microRNA profiling, making it a versatile tool for laboratories undertaking diverse studies or clinical research projects.

Streamlined Workflow and Reliable Results

With pre-mixed, ready-to-use reagents-including both reverse transcriptase and polymerase-and simple manual operation, the miRCURY LNA miRNA PCR Assay minimizes preparation time. It delivers accurate and consistent CT value outputs suitable for relative quantification, ensuring you achieve trustworthy insights into miRNA expression with minimal hassle.

FAQ's of Qiagen miRCURY LNA miRNA PCR Assay:

Q: How do I select the appropriate well format for my experiment?

A: You can choose from 12, 24, 60, or 96-well formats depending on your sample throughput needs. Smaller formats are ideal for pilot studies or a limited number of targets, while larger plates support high-throughput applications and comprehensive profiling.

Q: What sample types are compatible with the miRCURY LNA miRNA PCR Assay?

A: The assay is validated for a variety of biological materials including serum, plasma, tissue, cultured cells, urine, and other sample types, offering flexibility for different molecular research applications.

Q: When should the reagents be used by, and how should they be stored?

A: Use the reagents before the expiration date printed on the packaging, typically 12-24 months from manufacture. For optimal stability, store all reagents at -20C as recommended in the storage instructions.

Q: What equipment is required to operate this assay kit?

A: A compatible real-time PCR system, such as those from Roche, Applied Biosystems, or Bio-Rad, is required for running the assay and reading CT values. No additional power source is necessary as the kit is a consumable product.

Q: What is the process for detecting and quantifying miRNAs using this kit?

A: Extract total RNA from your biological sample, prepare the reaction by adding your RNA and kit reagents (including supplied primers and enzymes) into the wells, and run the plate on your real-time PCR instrument. The system generates CT values for each miRNA, allowing for relative quantification.

Q: What are the main benefits of using the miRCURY LNA miRNA PCR Assay?

A: This assay offers ultra-sensitive detection, high specificity due to LNA technology, pre-designed primers for ease of use, and robust results even from minimal RNA input. It's ideal for gene expression analysis, biomarker discovery, and validation, supporting both low- and high-throughput workflows.

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