Thermo Fisher Scientific Invitrogen PureLink DNase Set

Thermo Fisher Scientific Invitrogen PureLink DNase Set

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Product Details:

Function Removes contaminating genomic DNA during RNA purification
Usage Type Laboratory reagent
Shelf Life 1 year from date of manufacture (typical)
Features Efficient DNA removal, compatible with PureLink RNA kits, gentle on RNA, easy to use
Storage Instructions Store at -20C
Instruments Type Molecular biology reagent
Equipment Type DNase (Deoxyribonuclease) Set
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Thermo Fisher Scientific Invitrogen PureLink DNase Set Price And Quantity

Compatibility PureLink RNA Mini Kit and similar kits
Package Size Provided with 50 reactions per set
Catalog Number 12185010
Unit Definition 1 Kunitz unit degrades 1 µg of DNA in 10 min at 37°C
Form Lyophilized or liquid solution (as supplied)
RNase-Free Yes
Application RNA purification, RT-PCR, qRT-PCR, Northern blotting
Shipping Condition Shipped on dry ice
Hazardous Non-hazardous as per MSDS

Thermo Fisher Scientific Invitrogen PureLink DNase Set Product Specifications

Instruments Type Molecular biology reagent
Real-Time Operation No
Condition New
Storage Instructions Store at -20C
Dimension (L*W*H) Not specified (supplied in reagent vials)
Technology Recombinant enzyme technology
Features Efficient DNA removal, compatible with PureLink RNA kits, gentle on RNA, easy to use
Material Enzyme solution, buffer components
Use RNA purification and DNA removal from samples
Color Clear to slightly yellow (depends on buffer)
Wall Mounted No
Equipment Type DNase (Deoxyribonuclease) Set
Portable Yes
Usage Type Laboratory reagent
Function Removes contaminating genomic DNA during RNA purification
Operating Type Manual use
Shelf Life 1 year from date of manufacture (typical)

Compatibility PureLink RNA Mini Kit and similar kits
Package Size Provided with 50 reactions per set
Catalog Number 12185010
Unit Definition 1 Kunitz unit degrades 1 µg of DNA in 10 min at 37°C
Form Lyophilized or liquid solution (as supplied)
RNase-Free Yes
Application RNA purification, RT-PCR, qRT-PCR, Northern blotting
Shipping Condition Shipped on dry ice
Hazardous Non-hazardous as per MSDS

Product Description

Brand	Thermo Fisher Scientific
Product Type	DNase Kit
Pack Size	50 Preps
CAT No	12185010
Product Class	Molecular Biology

The RNase-free DNase set comes lyophilized and is optimized for on-column digestion of DNA using PureLink protocols. The degree of DNA digestion performed by PureLink DNase is optional for most PureLinka RNA kits, due to the efficient removal of the majority of DNA by the PureLink technology; however, some downstream applications that are sensitive to small amounts of DNA may require further removal of DNA.

Advanced DNA Removal for RNA Purity

The PureLink DNase Set leverages recombinant enzyme technology to deliver efficient DNA degradation during RNA purification. Its gentle formulation safeguards RNA, making it suitable for downstream processes that demand high RNA purity, like RT-PCR, qRT-PCR, and Northern blotting. This set provides confidence that your results will be free from DNA contamination.

Easy Integration with RNA Workflows

Designed for compatibility with PureLink RNA Mini Kit and comparable systems, the DNase Set offers seamless integration into standard laboratory protocols. Shipped on dry ice, the reagents (enzyme solution and buffer) arrive ready for manual use, requiring minimal preparation and user intervention. Its clear to slightly yellow color confirms reliability and consistency.

Reliable and Safe Laboratory Reagent

Intended for use in laboratory environments, the PureLink DNase Set is non-hazardous as per MSDS classifications, ensuring user safety. Its typical shelf life of one year supports efficient inventory management. Store the reagent at -20C to maintain enzyme activity for all 50 reactions in each set.

FAQ's of Thermo Fisher Scientific Invitrogen PureLink DNase Set:

Q: How do I use the PureLink DNase Set during RNA purification?

A: You can use the set by adding the provided DNase enzyme and buffer directly to your RNA sample after cell lysis and prior to RNA binding steps, following your kit's protocol. This ensures efficient removal of contaminating DNA before downstream applications.

Q: What applications benefit from using this DNase set?

A: The DNase set is particularly beneficial for RNA purification procedures preceding sensitive applications like RT-PCR, qRT-PCR, and Northern blotting, where DNA-free RNA is essential for accurate results.

Q: When should I perform DNase treatment in my RNA workflow?

A: DNase treatment should be executed after cell lysis but before RNA binding or elution during purification. Always refer to your RNA purification kit's specified steps for optimal timing.

Q: Where is the PureLink DNase Set compatible for use?

A: This set is compatible with the Invitrogen PureLink RNA Mini Kit and other similar RNA purification kits available in research and clinical laboratories.

Q: What process defines one Kunitz unit in this DNase set?

A: One Kunitz unit is defined as the amount of enzyme required to degrade 1 g of DNA in 10 minutes at 37C, ensuring consistency and reliable DNA removal during the treatment.

Q: Is the DNase set RNase-free, and how is this beneficial?

A: Yes, the set is RNase-free, preventing any degradation of RNA during DNA removal, which preserves RNA quality essential for downstream molecular biology techniques.

Q: What benefits does this DNase set provide over standard methods?

A: Compared to standard methods, it offers efficient and gentle DNA removal, is easy to use, highly compatible with RNA purification kits, and maintains the integrity of valuable RNA samples.

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