Qiagen Microbial DNA qPCR Assay Kit

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Product Details:

Measurement Range Wide dynamic range for microbial DNA quantification
Usage Type Laboratory
Accuracy Validated sensitivity with low copy number detection
Instruments Type Molecular biology kit
Features High sensitivity and specificity, compatible with any real-time PCR platform, ready-to-use master mix, validated primer sets
Function Quantitative amplification of microbial DNA targets
Shelf Life 12 months from date of manufacture
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Qiagen Microbial DNA qPCR Assay Kit Price And Quantity

Qiagen Microbial DNA qPCR Assay Kit Product Specifications

Measurement Range Wide dynamic range for microbial DNA quantification
Usage Type Laboratory
Technology Quantitative PCR (qPCR)
Color White (kit components)
Wall Mounted No
Use Detection and quantification of microbial DNA in samples
Operating Type Manual/Automated compatible
Real-Time Operation Yes
Accuracy Validated sensitivity with low copy number detection
Material Biological Reagents and Chemicals
Equipment Type Microbial DNA qPCR Assay Kit
Condition New
Instruments Type Molecular biology kit
Features High sensitivity and specificity, compatible with any real-time PCR platform, ready-to-use master mix, validated primer sets
Function Quantitative amplification of microbial DNA targets
Shelf Life 12 months from date of manufacture
Storage Instructions Store at -20C, avoid repeated freeze-thaw cycles

Product Description

Brand Qiagen

Product Type RT PCR Kit

Pack Size 20 l

CAT No 330033

Application Molecular Reagent

Microbial DNA qPCR Assay Kits are a complete solution for real-time PCR-based microbial detection. Microbial DNA qPCR Assay Kits contain a Microbial DNA qPCR Assay, which is a mix of two PCR primers (10 M each) and one 5 -hydrolysis probe (5 M) that enables quantitative real-time PCR for 20 reactions. The kit also contains everything required for a successful qPCR run, including Microbial DNA Positive Control, Positive PCR Control, Microbial DNA-Free Water, and Microbial qPCR Mastermix.
What is the difference between microbial identification and profiling? Identification is determining the microbes presence or absence in your sample which requires running a No Template Control during your analysis. Profiling is determining the microbes relative expression in two or more experimental conditions for which you will need to run a reference sample and a normalizer (provided by QIAGEN).

Versatile Microbial DNA Quantification

The Qiagen Microbial DNA qPCR Assay Kit facilitates detection and quantification of microbial DNA from diverse clinical, environmental, and food sources. By offering both TaqMan-type probe and dye-based options, this kit adapts to a variety of research and testing needs, making it invaluable in laboratories focused on microbial surveillance, food safety, and diagnostic applications.

Universal Compatibility and High Sensitivity

This kit is engineered for seamless integration with virtually any real-time PCR cycler, including Applied Biosystems, Bio-Rad, and Qiagen Rotor-Gene platforms. Its high sensitivity is validated down to low copy numbers, while the included controls and ready-to-use reagents ensure reliable and reproducible results, even for complex sample matrices.

Reliable, Ready-to-Use Reagents

Each kit comes complete with a qPCR master mix, validated primer/probe mixes, positive and negative control DNAs, and nuclease-free water, making setup and execution straightforward. The streamlined process reduces preparation time and minimizes handling errors, supporting robust microbial DNA quantification for a range of laboratory workflows.

FAQs of Qiagen Microbial DNA qPCR Assay Kit:

Q: How do I use the Qiagen Microbial DNA qPCR Assay Kit for microbial detection?

A: The kit is designed for quantitative detection of microbial DNA. Simply combine your purified microbial DNA sample with the provided master mix and primer/probe mix, then run the assay on a compatible real-time PCR cycler following the user manual. Positive and negative controls are included to validate your results.

Q: What types of samples are suitable for this assay kit?

A: You can use purified microbial DNA from a variety of sources, including clinical, food, and environmental samples. Ensure that your DNA is properly extracted and of high quality for optimal qPCR performance.

Q: When should I store the kit components and how should I handle them?

A: All kit components should be stored at -20C immediately upon receipt. Avoid repeated freeze-thaw cycles to maintain reagent integrity and ensure reliable results throughout the 12-month shelf life.

Q: Where can the Qiagen Microbial DNA qPCR Assay Kit be used?

A: This kit is suitable for use in research labs, food testing facilities, environmental monitoring stations, and clinical microbiology laboratories across India and globally wherever robust microbial quantification is required.

Q: What is the benefit of using a probe-based (TaqMan) versus dye-based assay format?

A: Probe-based (TaqMan) assays offer higher specificity due to the presence of a sequence-specific probe, reducing the chance of non-specific amplification, while dye-based assays provide simplicity and cost-effectiveness. The kit configuration determines which method you use, and both are validated for sensitivity and accuracy.

Q: Is the kit compatible with automated systems or manual workflows?

A: Yes, the Qiagen Microbial DNA qPCR Assay Kit can be used with both manual and automated workflows, allowing flexibility in sample processing and PCR setup for different laboratory throughput requirements.

Q: How does the kit ensure accuracy and reproducibility in results?

A: Validated primer sets, positive and negative controls, and a robust master mix within the kit help guarantee precision and reproducibility in quantifying microbial targets, even at low DNA concentrations. Its wide dynamic range is especially advantageous for variable sample types.

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