Product Description
Brand Qiagen
Product Type PCR Kit
Pack Size 5 ml
CAT No 250102
Application Molecular Reagent
The QIAcuity Probe PCR Kit contains a 4x concentrated, ready to use Master Mix optimized for microfluidic use in theQIAcuity Nanoplates. The kit enhances the specificity and efficiency of probe-based digital PCR to provide accurate, singleplex, or up to 5-plex analysis. The QIAcuity Probe Master Mix is optimized to increase specificity for accurate quantification of gDNA or cDNA on the QIAcuity dPCR instruments.
Optimized Performance with QIAcuity SystemsThe QIAcuity Probe PCR Kit is engineered for seamless integration with QIAcuity Digital PCR Systems, ensuring high amplification fidelity and robust quantitative results. Its innovative probe chemistry supports accurate multiplex analysis, making it well suited for complex research workflows in molecular biology laboratories.
Reliability and Convenience in Every StepWith ready-to-use reagents, quality controls, and detailed protocols, the QIAcuity Probe PCR Kit streamlines your workflow. Lot number traceability enhances quality assurance, while the kit's optimized format and comprehensive instructions help facilitate consistent, reproducible results in every run.
FAQ's of Qiagen QIAcuity Probe PCR Kit (5 mL):
Q: How do I store the QIAcuity Probe PCR Kit to maintain its stability?
A: The kit should be stored at -15C to -25C. Following the recommended storage conditions ensures reagent stability and maximizes the 18-month shelf life from the manufacturing date.
Q: What benefits does the QIAcuity Probe PCR Kit offer in digital PCR applications?
A: This kit delivers high sensitivity, accurate quantification, and a wide dynamic range, making it ideal for both qualitative and quantitative detection of nucleic acids in research. It is specifically optimized for use with QIAcuity dPCR systems for trusted results.
Q: When is it appropriate to use this kit for experiments?
A: The QIAcuity Probe PCR Kit is intended for laboratory research purposes whenever sensitive detection and the quantification of DNA or RNA are required, such as gene expression studies or mutation analysis. It is not for diagnostic or clinical applications.
Q: What is included in the kit package and what is the intended sample input volume?
A: The kit contains a 5 mL PCR master mix, relevant controls, and a protocol insert. It is formulated for use with up to 5 L of DNA/RNA template per reaction, delivering convenience and compatibility with nanoplates (8.5 to 40 L final reaction volume).
Q: How does the QIAcuity Probe PCR Kit achieve accurate detection?
A: It uses hot-start DNA polymerase and TaqMan hydrolysis probe chemistry to provide precise target amplification and sensitive detection. The reagents are designed for high accuracy and reproducibility in digital PCR analyses.
Q: Where can I use this kit and what equipment is required?
A: Designed for laboratory research in India and worldwide, the QIAcuity Probe PCR Kit must be used with the QIAcuity Digital PCR System as it is a consumable specifically optimized for these analyzers. No standalone equipment is supplied.