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Qiagen QuantiTect Rev. Transcription Kit (50) Qiagen QuantiTect Rev. Transcription Kit (50) Qiagen QuantiTect Rev. Transcription Kit (50)
Qiagen QuantiTect Rev. Transcription Kit (50)
Qiagen QuantiTect Rev. Transcription Kit (50) Qiagen QuantiTect Rev. Transcription Kit (50)

Qiagen QuantiTect Rev. Transcription Kit (50)

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Product Details:

  • Features Integrated removal of genomic DNA contamination; Efficient and sensitive cDNA synthesis; Ready-to-use Master Mix; One-step protocol
  • Storage Instructions Store at -20C
  • Usage Type Laboratory
  • Instruments Type Molecular Biology/Genomics Reagent Kit
  • Function Reverse transcription of total RNA, mRNA, or viral RNA
  • Shelf Life 12 months
  • Accuracy High-fidelity cDNA synthesis
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Qiagen QuantiTect Rev. Transcription Kit (50) Price And Quantity

Qiagen QuantiTect Rev. Transcription Kit (50) Product Specifications

  • No
  • Reagents and Enzymes in Solution
  • New
  • Store at -20C
  • Integrated removal of genomic DNA contamination; Efficient and sensitive cDNA synthesis; Ready-to-use Master Mix; One-step protocol
  • Manual
  • For synthesis of cDNA from RNA templates
  • Colorless (liquid reagents)
  • Reverse Transcription Kit
  • 12 months
  • Reverse Transcription (RT) of RNA to cDNA
  • Reverse transcription of total RNA, mRNA, or viral RNA
  • Molecular Biology/Genomics Reagent Kit
  • Laboratory
  • Yes (Compatible with real-time PCR)
  • High-fidelity cDNA synthesis

Product Description

Brand Qiagen

Product Type Transcription Kit

Pack Size 50 & 200 T

CAT No 205311, 205313

Application Molecular Reagent



The unique QuantiTect Reverse Transcription Kit provides a fast and convenient procedure for cDNA synthesis with integrated genomic DNA removal. Genomic DNA contamination in RNA samples is effectively eliminated by gDNA Wipeout Buffer. All components that are required for fast and efficient reverse transcription are provided with the QuantiTect Reverse Transcription Kit, including Quantiscript Reverse Transcriptase, Quantiscript RT Buffer, and a unique RT Primer Mix. The synthesized cDNA is optimized for use in real-time PCR, allowing reliable quantification of targets from all regions of mRNA transcripts.




Efficient & Reliable cDNA Synthesis

Qiagen's QuantiTect Rev. Transcription Kit streamlines cDNA production from diverse RNA sources, thanks to its robust enzyme blend and integrated genomic DNA removal. The ready-to-use master mix ensures consistency and reduces handling steps, providing reliable results for subsequent RT-PCR or real-time RT-PCR applications. High cDNA yields and sensitivity support a broad range of molecular biology experiments.


User-Friendly One-Step Protocol

The kit enables simplified cDNA synthesis in a single protocol, reducing potential errors and hands-on time. With everything needed for up to 50 reactions included, users can quickly set up their reverse transcription reactions with minimal preparation. Heat-inactivation is not required, making the process even more convenient for routine laboratory workflows.


Quality and Compatibility Assurance

Each kit undergoes stringent quality control to confirm the absence of RNase and DNase contamination, ensuring the integrity of your RNA samples. cDNA generated with this kit is fully compatible with downstream PCR and real-time PCR platforms, making it a versatile choice for laboratories engaging in molecular genetics research, viral detection, or gene expression analysis.

FAQ's of Qiagen QuantiTect Rev. Transcription Kit (50):


Q: How do I use the Qiagen QuantiTect Rev. Transcription Kit for cDNA synthesis?

A: Simply combine your RNA template (10 pg-1 g per reaction) with the provided reagents, following the one-step protocol. The kit's integrated gDNA Wipeout Buffer eliminates genomic DNA contamination, while the ready-to-use enzyme mix efficiently synthesizes cDNA at 42C in 20 minutes. No heat inactivation step is required.

Q: What applications is this kit suited for in the laboratory?

A: This kit is optimized for RT-PCR and real-time RT-PCR, facilitating cDNA synthesis from total RNA, mRNA, or viral RNA for downstream gene expression studies or molecular diagnostics.

Q: When should I use the gDNA Wipeout Buffer included in the kit?

A: The gDNA Wipeout Buffer is used at the beginning of the protocol, before reverse transcription. It removes contaminating genomic DNA to prevent interference in PCR assays, ensuring reliable amplification from only cDNA.

Q: Where should I store the kit components for optimal performance?

A: All liquid reagents and enzymes should be stored at -20C as directed. Proper storage helps maintain enzyme activity and guarantees the kit's shelf life of up to 12 months.

Q: What benefits does the QuantiTect Rev. Transcription Kit offer over standard reverse transcription methods?

A: The kit provides high-fidelity cDNA synthesis with an integrated DNA removal step and a ready-to-use master mix format, reducing protocol complexity and the risk of sample contamination while delivering consistently high yields.

Q: Is this kit compatible with real-time PCR instruments and downstream PCR applications?

A: Yes, cDNA generated using the QuantiTect kit is fully compatible with both standard and real-time PCR platforms, supporting a variety of molecular biology workflows.

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