Qiagen REPLI-g Single Cell Kit (96)

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Product Details:

Accuracy High genomic coverage, consistent amplification
Features High genomic coverage, minimal DNA sequence bias, ready to use, high reproducibility
Instruments Type Molecular Biology Reagent Kit
Storage Instructions Store at -20C
Function Whole Genome Amplification (WGA)
Usage Type Laboratory
Measurement Range Single cell DNA to nanogram levels
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Qiagen REPLI-g Single Cell Kit (96) Price And Quantity

Qiagen REPLI-g Single Cell Kit (96) Product Specifications

Color White/Transparent (Reagents)
Instruments Type Molecular Biology Reagent Kit
Portable Yes (Reagent Kit)
Operating Type Manual
Accuracy High genomic coverage, consistent amplification
Features High genomic coverage, minimal DNA sequence bias, ready to use, high reproducibility
Usage Type Laboratory
Wall Mounted No
Function Whole Genome Amplification (WGA)
Storage Instructions Store at -20C
Shelf Life 12 months
Power Source Not Required (Enzymatic Reaction)
Measurement Range Single cell DNA to nanogram levels
Material Reagents and Enzymes (including buffers, polymerase, control DNA)
Technology Multiple Displacement Amplification (MDA)
Real-Time Operation No
Equipment Type Single Cell Whole Genome Amplification Kit
Condition New
Use Amplification of genomic DNA from single or a few cells

Product Description

Brand Qiagen

Product Type PCR Kit

Pack Size 96 Wells

CAT No 150345

Application Molecular Reagent

DNA sequence analysis and genotyping of biological samples using innovative instrumentation, such as next-generation sequencing (NGS) platforms, is often limited by the small amount of sample available. The REPLI-g Single Cell Kit is specially designed to uniformly amplify genomic DNA from single cells (1 to <1000 bacterial or tumor cells) or purified genomic DNA with complete genome coverage. Additional protocols are also available for use with fresh or dried blood or fresh or frozen tissue. Dedicated buffers and reagents undergo a unique, controlled decontamination procedure to avoid amplification of contaminating DNA, ensuring highly reliable results every time. Accurate amplification of genomes with negligible sequence bias and no genomic drop-outs is achieved with innovative Multiple Displacement Amplification (MDA) technology. In contrast to PCR-based WGA technologies, high fidelity rates are increased up to 1000-fold, avoiding costly false positive or negative results.

Efficient Single Cell DNA Amplification

With the REPLI-g Single Cell Kit, genomic DNA from as little as a single cell can be successfully amplified to nanogram quantities. Using Phi29 DNA Polymerase and specialized reagents, gain high-fidelity results with minimal sequence bias, making this kit an essential choice for researchers needing consistent and comprehensive genomic coverage from low-input DNA sources.

Ready-to-Use Kit for Versatile Applications

Each kit provides all the necessary reagents and enzymes for manual, whole-genome amplification workflows. Whether your work involves cultured, sorted, or micromanipulated cells, the kit fits seamlessly into laboratory pipelines for genomics, diagnostics, or forensic tasks. The easy protocol and reliable results are perfect for varied laboratory needs.

Comprehensive Kit Components and Protocol

Included in the kit are the REPLI-g sc Reaction Buffer, DNA Polymerase, denaturation and lysis buffers, control DNA, and nuclease-free water. These components work together using Multiple Displacement Amplification (MDA) technology, allowing for robust DNA yield and excellent reproducibility. All reagents are colorless to ensure straightforward reaction monitoring and handling.

FAQ's of Qiagen REPLI-g Single Cell Kit (96):

Q: How does the REPLI-g Single Cell Kit work for whole genome amplification?

A: The kit utilizes Multiple Displacement Amplification (MDA) with Phi29 DNA Polymerase and optimized buffers to amplify genomic DNA from single cells, generating up to 40 g DNA per reaction in under 8 hours.

Q: What sample types are compatible with the kit?

A: The kit is suitable for cultured cells, sorted cells, and micromanipulated cells, allowing flexibility in sample origin for genomic DNA amplification from minimal starting material.

Q: When should I use this kit instead of standard DNA extraction methods?

A: Use this kit when working with single cells or very low DNA quantities where standard extraction and amplification methods may be insufficient for downstream applications that require comprehensive genomic coverage.

Q: Where can the REPLI-g Single Cell Kit be used?

A: It is intended for use in laboratory settings and is suitable for genomics research, pre-implantation genetic diagnosis, forensic analysis, and any application requiring whole genome amplification from single or few cells.

Q: What is the process for using the kit?

A: The workflow involves cell lysis, genomic DNA denaturation, and isothermal amplification with ready-to-use reagents included in the kit. The entire process completes in less than 8 hours.

Q: How should I store the kit and what is its shelf life?

A: Store all reagents at -20C. The kit maintains optimal performance for up to 12 months if properly stored.

Q: What are the key benefits of using the REPLI-g Single Cell Kit?

A: Benefits include high genomic coverage, minimal sequence bias, robust DNA yields from single cells, and reliable, reproducible results, making it highly suitable for sensitive single-cell and low-input DNA workflows.

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