Product Description
Brand Waters Corp
Sample Type Reagent
Pack Size 20 mL
CAT No WAT084189
Application Protein
Paired -Ion Chromatography (PIC Reagents) provide an easy solution for analyzing samples containing ionic species. These reagents allow ionic compounds to be separated by reverse-phase chromatography, saving time by allowing simultaneous assay of acids, bases, amphoteric and neutral compounds. PIC A Low UV is 5 vials/pkg of tertrabutylammonium hydrogen sulfate in a workable UV range in mobile phase 200+ nm.
Optimized for Low UV DetectionPIC A Low UV Reagent stands out for its minimal UV absorbance, making it particularly suitable for analytical methods detecting at low wavelengths. This characteristic ensures sensitive detection without interference, supporting precise ion-pair chromatography in HPLC applications.
Convenient and Safe HandlingPackaged in an opaque HDPE plastic bottle, the reagent is protected from light exposure, maintaining its stability over a 24-month unopened shelf life. Its non-hazardous and non-flammable nature removes complicated safety requirements, streamlining laboratory usage.
Consistent Performance for Accurate ResultsThe proprietary phosphate buffer formulation (pH 6.0) delivers high reproducibility in anion and cation analysis. It is ready-to-use, requires no further dilution or preparation, and is compatible with a range of HPLC systems, facilitating seamless workflow integration for reliable outcomes.
FAQ's of Waters Corp PIC A Low UV Reagent:
Q: How do I use the Waters Corp PIC A Low UV Reagent in my HPLC workflow?
A: Simply pour the ready-to-use reagent directly from the sealed opaque HDPE bottle into your HPLC reservoir. It is optimized for ion-pair chromatography and suitable for reverse-phase HPLC analysis of anions and cations, requiring no further preparation.
Q: What makes this reagent suitable for low wavelength UV detection?
A: This buffer is specifically formulated for minimal UV absorbance, allowing accurate detection at low wavelengths. Its low UV background ensures your HPLC results reflect true analyte signals, not absorption from the mobile phase.
Q: When should I replace the reagent bottle?
A: The unopened reagent has a 24-month shelf life. Replace the reagent if it has passed its expiration date or if contamination or discoloration is observed, even within the date range.
Q: Where should I store the PIC A Low UV Reagent for best stability?
A: Store the bottle at room temperature, ideally between 15-30C, and keep it in its opaque container to protect the phosphate buffer from light exposure, preserving the reagent's performance.
Q: What are the benefits of using this reagent in ion-pair chromatography?
A: PIC A Low UV Reagent offers high reproducibility and minimizes UV absorption, which enhances signal-to-noise ratios, reduces background interference, and improves the accuracy of anion and cation detection in your HPLC analyses.
Q: Is any special handling required for this reagent?
A: No, the reagent is non-hazardous and non-flammable. Standard laboratory precautions for aqueous buffers are sufficient, and no special disposal or storage regulations apply.