Qiagen DyeEx 96 Kit (24)

Get a Price/Quote

Product Details:

Usage Type Laboratory
Measurement Range For clean-up of up to 10 l dye-terminator sequencing reactions per well
Accuracy Efficient removal of dye terminators with minimal sample loss
Instruments Type Nucleic acid purification equipment
Storage Instructions Store at room temperature (1525C)
Function Purification of labeled DNA sequencing products
Features High-throughput processing, efficient dye removal, rapid protocol
Click to View more
Share Your Product:

Request to Call Back Send Inquiry

Qiagen DyeEx 96 Kit (24) Price And Quantity

Procedure Time Approximately 10 minutes
Throughput 96 samples per run
Kit Content DyeEx 96 plates, collection plates, protocols
Sample Type Dye-labeled DNA products from sequencing
Elution Volume 40 µl per well
Catalog Number 63183
Capacity per Kit 24 × 96 reactions
Application Sanger sequencing, fluorescent sequencing reaction clean-up
Sample Volume Compatibility 10 µl per well

Qiagen DyeEx 96 Kit (24) Product Specifications

Real-Time Operation No
Use Removal of unincorporated dye terminators from sequencing reactions
Equipment Type DNA Purification Kit
Weight Approx. 1.1 kg per kit
Function Purification of labeled DNA sequencing products
Shelf Life 12 months
Noise Level Silent operation
Features High-throughput processing, efficient dye removal, rapid protocol
Color Transparent/White
Usage Type Laboratory
Dimension (L*W*H) Standard microplate size (approx. 127.8 85.5 mm)
Instruments Type Nucleic acid purification equipment
Technology Spin-column and gel filtration
Operating Type Manual
Wall Mounted No
Portable Yes
Condition New
Accuracy Efficient removal of dye terminators with minimal sample loss
Material High-quality plastic (96-well plate and collection plate)
Storage Instructions Store at room temperature (1525C)
Measurement Range For clean-up of up to 10 l dye-terminator sequencing reactions per well

Procedure Time Approximately 10 minutes
Throughput 96 samples per run
Kit Content DyeEx 96 plates, collection plates, protocols
Sample Type Dye-labeled DNA products from sequencing
Elution Volume 40 µl per well
Catalog Number 63183
Capacity per Kit 24 × 96 reactions
Application Sanger sequencing, fluorescent sequencing reaction clean-up
Sample Volume Compatibility 10 µl per well

Product Description

Brand Qiagen

Product Type DyeEx

Pack Size 24 x 96 Wells

CAT No 63183

Application Molecular Reagent

DyeEx Kits provide either spin columns or 96-well plates and use gel-filtration technology to remove dye terminators from sequencing reactions, including reactions for Sanger sequencing. Sequencing reactions are loaded onto the pre-hydrated gel-filtration material. After a short centrifugation step, the reactions are ready to be loaded onto a capillary sequencer. Unincorporated dye terminators are retained in the gel matrix.

High-Throughput Sequencing Clean-Up

The Qiagen DyeEx 96 Kit (24) allows laboratories to process up to 96 sequencing samples per plate, significantly increasing throughput and laboratory efficiency. Its fast procedure and high-capacity design are well-suited for projects demanding reliable purification of dye-labeled DNA products. This kit streamlines workflows for Sanger and fluorescent sequencing clean-up tasks.

Efficient and Reliable Dye Removal

Utilizing a spin-column and gel filtration technology, this kit ensures effective removal of unincorporated dye terminators from sequencing reactions. The protocol is designed to minimize sample loss and ensure high-purity DNA, making it ideal for sensitive downstream applications that rely on accurate sequencing results.

Simplified and Rapid Workflow

The DyeEx 96 Kit offers a rapid protocol, completing clean-up in approximately 10 minutes. Manual, straightforward operation makes it easy for users to adopt without specialized training. Its standard microplate dimensions and silent operation integrate seamlessly into most laboratory setups, ensuring an effortless purification experience.

FAQ's of Qiagen DyeEx 96 Kit (24):

Q: How does the Qiagen DyeEx 96 Kit purify dye-labeled DNA from sequencing reactions?

A: The Qiagen DyeEx 96 Kit uses a combination of spin-column and gel filtration technology to efficiently remove unincorporated dye terminators from dye-labeled DNA, resulting in clean products suitable for Sanger or fluorescent sequencing analysis.

Q: What is the maximum sample volume that can be processed per well with this kit?

A: Each well of the DyeEx 96 plate can accommodate up to 10 l of dye-terminator sequencing reaction, with elution carried out in 40 l per well for optimal DNA recovery.

Q: When should I use the DyeEx 96 Kit in my sequencing workflow?

A: Use the DyeEx 96 Kit after dye-terminator sequencing reactions, right before the sequencing analysis step. It is specifically designed to remove excess dye terminators to ensure accurate and clear sequencing results.

Q: Where can this kit be used, and is it portable?

A: This kit is suitable for laboratory environments across dealer, distributor, exporter, manufacturer, supplier, trader, and wholesaler settings in India. It is lightweight, portable, and fits into standard microplate-compatible instruments.

Q: What are the main steps in the clean-up process using this kit?

A: The process involves loading the sequencing sample into each well, spinning the plate to separate and capture purified DNA, and collecting the eluate in the supplied collection plate, following the provided protocol. Total clean-up time is around 10 minutes.

Q: What are the advantages of using the Qiagen DyeEx 96 Kit?

A: Key benefits include high-throughput processing (96 samples/run), fast and efficient dye removal with minimal sample loss, and user-friendly operation without generating noise. Results are reliable and highly reproducible, supporting accurate sequencing analysis.

Q: How should I store the Qiagen DyeEx 96 Kit, and what is its shelf life?

A: Store the kit at room temperature (15-25C). The shelf life is 12 months from the date of purchase if stored under recommended conditions.

Tell us about your requirement

Price:

Quantity

Select Unit

50
100
200
250
500
1000+

Additional detail

Mobile number

Other Products in 'Medical Reagents' category

Mindray Creatine kinase MB (CLIA) Wall Mounted : No Operating Type : Other, Automated Instruments Type : Reagent for CL2000i, CL1200i and compatible Mindray CLIA analyzers Technology : Chemiluminescence Immunoassay (CLIA) Usage Type : Invitro Diagnostic (IVD) Measurement Range : Specifically targets CKMB isoenzyme. Check analyzer manual for analytical range. Storage Instructions : 28C, protect from light Accuracy : High accuracy, consistent performance Condition : New Material : Other, Diagnostic Kit, Reagent Liquid Portable : No Function : Quantitative detection of creatine kinaseMB (CKMB) Use : Creatine kinase MB detection in human serum/plasma Features : High sensitivity, High stability, Readytouse liquid reagents, Reliable results Shelf Life : 12 months from manufacturing date Equipment Type : Clinical Laboratory Analyzer Reagent Real-Time Operation : Yes Color : Reagent (Liquid) Clear / Light Tone, Kit Packaging White with details Dimension (L*W*H) : Kit/box size not specified Weight : Kit weight not specified Power Source : Other, Not Applicable (used with analyzer)

Send Inquiry

Qiagen QIAseq FX DNA Library Kit HT A-D (384) Wall Mounted : No Operating Type : Manual Instruments Type : NGS Library Preparation Kit Technology : Enzymatic Fragmentation and Library Prep (NGS) Usage Type : Laboratory use Measurement Range : Compatible with DNA input 10 ng 1 g Storage Instructions : Store at 20C Accuracy : High fidelity, minimizes sequence bias Condition : New Material : Other, Reagents, Enzyme Mix, Buffers, Solution, Tubes Portable : Yes Function : Simultaneous DNA fragmentation and library prep Use : Preparation of DNA libraries for NextGeneration Sequencing Features : Highthroughput, enzymatic fragmentation, rapid protocol, high yield, compatible with Illumina platforms Shelf Life : 12 months from manufacturing date Equipment Type : DNA Library Preparation Kit Real-Time Operation : No Color : Multicolor (as per reagent) Dimension (L*W*H) : Kit dimensions vary; standard laboratory kit size Weight : Approx. 1 kg (shipping weight) Power Source : Other, Not required (compatible with common laboratory thermal cycler)

Send Inquiry

Thermo Fisher Scientific Invitrogen Platinum SYBR Green qPCR SuperMix-UDG Wall Mounted : No Operating Type : Other, Readytouse master mix for realtime PCR Instruments Type : Compatible with most realtime PCR instruments Technology : RealTime PCR with SYBR Green I dye detection Usage Type : Laboratory/Research Measurement Range : Dynamic range typically: 68 orders of magnitude (depending on instrument and assay) Storage Instructions : Store at 20 C for longterm stability Accuracy : High sensitivity and specificity for qPCR applications Condition : New Material : Other, Liquid reagent; primarily buffer and enzyme solution Portable : Yes (reagent format) Function : Enables sensitive DNA or cDNA quantification via realtime PCR Use : Quantitative PCR (qPCR) and quantitative reversetranscription PCR (qRTPCR) for DNA and cDNA target detection Features : Contains Platinum Taq DNA Polymerase, SYBR Green I dye for doublestranded DNA detection, UDG (uracilDNA glycosylase) for prevention of carryover contamination, optimized buffer system, high sensitivity Shelf Life : 12 months (when stored properly) Equipment Type : qPCR SuperMix Real-Time Operation : Yes Color : Light green (due to SYBR Green dye) Dimension (L*W*H) : Not applicable (reagent bottle/tube format) Power Source : Other, Not required (used as a reagent in compatible qPCR cyclers)

Send Inquiry

Mindray Troponin I (CLIA) Wall Mounted : No Operating Type : Other, Automated Instruments Type : Compatible with Mindray CL900i, CL6000i, CL8000i analyzers Technology : Chemiluminescence Immunoassay (CLIA) Usage Type : Hospital, Laboratory Measurement Range : 0.0150 ng/mL Storage Instructions : Store at 28C, away from direct sunlight Accuracy : 10% CV at decision point Condition : New Material : Other, Biological reagents Portable : No Function : Detects cardiac troponin I Use : Quantitative determination of cardiac troponin I Features : High sensitivity, rapid result, compatible with Mindray CL series analyzers Shelf Life : 12 months Equipment Type : Immunoassay Reagent Kit Real-Time Operation : Yes

Send Inquiry

SEND INQUIRY CALL ME FREE