Qiagen DyeEx 96 Kit (24)

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Product Details:

Function Purification of labeled DNA sequencing products
Accuracy Efficient removal of dye terminators with minimal sample loss
Features High-throughput processing, efficient dye removal, rapid protocol
Measurement Range For clean-up of up to 10 l dye-terminator sequencing reactions per well
Shelf Life 12 months
Storage Instructions Store at room temperature (1525C)
Instruments Type Nucleic acid purification equipment
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Qiagen DyeEx 96 Kit (24) Price And Quantity

Qiagen DyeEx 96 Kit (24) Product Specifications

Technology Spin-column and gel filtration
Noise Level Silent operation
Accuracy Efficient removal of dye terminators with minimal sample loss
Features High-throughput processing, efficient dye removal, rapid protocol
Color Transparent/White
Material High-quality plastic (96-well plate and collection plate)
Portable Yes
Function Purification of labeled DNA sequencing products
Wall Mounted No
Weight Approx. 1.1 kg per kit
Usage Type Laboratory
Operating Type Manual
Instruments Type Nucleic acid purification equipment
Condition New
Measurement Range For clean-up of up to 10 l dye-terminator sequencing reactions per well
Dimension (L*W*H) Standard microplate size (approx. 127.8 85.5 mm)
Real-Time Operation No
Equipment Type DNA Purification Kit
Shelf Life 12 months
Storage Instructions Store at room temperature (1525C)
Use Removal of unincorporated dye terminators from sequencing reactions

Product Description

Brand Qiagen

Product Type DyeEx

Pack Size 24 x 96 Wells

CAT No 63183

Application Molecular Reagent

DyeEx Kits provide either spin columns or 96-well plates and use gel-filtration technology to remove dye terminators from sequencing reactions, including reactions for Sanger sequencing. Sequencing reactions are loaded onto the pre-hydrated gel-filtration material. After a short centrifugation step, the reactions are ready to be loaded onto a capillary sequencer. Unincorporated dye terminators are retained in the gel matrix.

High-Throughput Sequencing Clean-Up

The Qiagen DyeEx 96 Kit (24) allows laboratories to process up to 96 sequencing samples per plate, significantly increasing throughput and laboratory efficiency. Its fast procedure and high-capacity design are well-suited for projects demanding reliable purification of dye-labeled DNA products. This kit streamlines workflows for Sanger and fluorescent sequencing clean-up tasks.

Efficient and Reliable Dye Removal

Utilizing a spin-column and gel filtration technology, this kit ensures effective removal of unincorporated dye terminators from sequencing reactions. The protocol is designed to minimize sample loss and ensure high-purity DNA, making it ideal for sensitive downstream applications that rely on accurate sequencing results.

Simplified and Rapid Workflow

The DyeEx 96 Kit offers a rapid protocol, completing clean-up in approximately 10 minutes. Manual, straightforward operation makes it easy for users to adopt without specialized training. Its standard microplate dimensions and silent operation integrate seamlessly into most laboratory setups, ensuring an effortless purification experience.

FAQ's of Qiagen DyeEx 96 Kit (24):

Q: How does the Qiagen DyeEx 96 Kit purify dye-labeled DNA from sequencing reactions?

A: The Qiagen DyeEx 96 Kit uses a combination of spin-column and gel filtration technology to efficiently remove unincorporated dye terminators from dye-labeled DNA, resulting in clean products suitable for Sanger or fluorescent sequencing analysis.

Q: What is the maximum sample volume that can be processed per well with this kit?

A: Each well of the DyeEx 96 plate can accommodate up to 10 l of dye-terminator sequencing reaction, with elution carried out in 40 l per well for optimal DNA recovery.

Q: When should I use the DyeEx 96 Kit in my sequencing workflow?

A: Use the DyeEx 96 Kit after dye-terminator sequencing reactions, right before the sequencing analysis step. It is specifically designed to remove excess dye terminators to ensure accurate and clear sequencing results.

Q: Where can this kit be used, and is it portable?

A: This kit is suitable for laboratory environments across dealer, distributor, exporter, manufacturer, supplier, trader, and wholesaler settings in India. It is lightweight, portable, and fits into standard microplate-compatible instruments.

Q: What are the main steps in the clean-up process using this kit?

A: The process involves loading the sequencing sample into each well, spinning the plate to separate and capture purified DNA, and collecting the eluate in the supplied collection plate, following the provided protocol. Total clean-up time is around 10 minutes.

Q: What are the advantages of using the Qiagen DyeEx 96 Kit?

A: Key benefits include high-throughput processing (96 samples/run), fast and efficient dye removal with minimal sample loss, and user-friendly operation without generating noise. Results are reliable and highly reproducible, supporting accurate sequencing analysis.

Q: How should I store the Qiagen DyeEx 96 Kit, and what is its shelf life?

A: Store the kit at room temperature (15-25C). The shelf life is 12 months from the date of purchase if stored under recommended conditions.

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