Thermo Fisher Scientific Applied Biosystems PowerTrack SYBR Green Master Mix for qPCR

Thermo Fisher Scientific Applied Biosystems PowerTrack SYBR Green Master Mix for qPCR

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Product Details:

Features Dual-track hot-start, high specificity, superior sensitivity, lot-to-lot consistency, compatible with all Applied Biosystems real-time PCR instruments, ROX normalization
Measurement Range Wide dynamic range suitable for low- and high-abundance targets
Shelf Life 12 months
Instruments Type Compatible with real-time PCR thermal cyclers
Accuracy Highoptimized for reproducibility and specificity
Usage Type Laboratory reagent
Function Enables quantitative DNA amplification and detection in qPCR
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Thermo Fisher Scientific Applied Biosystems PowerTrack SYBR Green Master Mix for qPCR Price And Quantity

Application Gene expression analysis, copy number variation, genotyping, DNA quantification
Inhibitor Tolerance High (compatible with complex samples)
Performance Highly sensitive detection with minimal primer-dimer formation
Recommended Reaction Volume 1050 L
Reference Dye Passive ROX Reference Dye included for normalization
Product Name Applied Biosystems PowerTrack SYBR Green Master Mix
Compatible Instruments Applied Biosystems 7500, QuantStudio, StepOnePlus, and all major qPCR platforms
Enzyme Dual hot-start DNA polymerase
Certificates/Compliance ISO 9001 certified manufacturing
Packaging Bottle/vial (typically 2 mL or 5 mL format)
Sample Types Genomic DNA, cDNA, plasmid DNA, PCR amplicons
Detection SYBR Green I dye fluorescence, detects double-stranded DNA

Thermo Fisher Scientific Applied Biosystems PowerTrack SYBR Green Master Mix for qPCR Product Specifications

Storage Instructions Store at -20C; avoid repeated freeze-thaw cycles
Operating Type Automated with qPCR instrument
Real-Time Operation Yes
Color Green (dye) / Clear solution
Function Enables quantitative DNA amplification and detection in qPCR
Equipment Type qPCR Master Mix
Technology Real-Time PCR (qPCR) with SYBR Green I chemistry
Usage Type Laboratory reagent
Portable Yes (reagent vials/tubes)
Wall Mounted No
Dimension (L*W*H) 2 mL and 5 mL tube formats
Accuracy Highoptimized for reproducibility and specificity
Condition New
Instruments Type Compatible with real-time PCR thermal cyclers
Shelf Life 12 months
Use Quantitative PCR (qPCR) for gene expression and analysis
Features Dual-track hot-start, high specificity, superior sensitivity, lot-to-lot consistency, compatible with all Applied Biosystems real-time PCR instruments, ROX normalization
Measurement Range Wide dynamic range suitable for low- and high-abundance targets
Material Proprietary reagent formulation (includes SYBR Green dye, antibodies, buffer components, dNTPs, ROX Reference Dye)

Application Gene expression analysis, copy number variation, genotyping, DNA quantification
Inhibitor Tolerance High (compatible with complex samples)
Performance Highly sensitive detection with minimal primer-dimer formation
Recommended Reaction Volume 1050 L
Reference Dye Passive ROX Reference Dye included for normalization
Product Name Applied Biosystems PowerTrack SYBR Green Master Mix
Compatible Instruments Applied Biosystems 7500, QuantStudio, StepOnePlus, and all major qPCR platforms
Enzyme Dual hot-start DNA polymerase
Certificates/Compliance ISO 9001 certified manufacturing
Packaging Bottle/vial (typically 2 mL or 5 mL format)
Sample Types Genomic DNA, cDNA, plasmid DNA, PCR amplicons
Detection SYBR Green I dye fluorescence, detects double-stranded DNA

Product Description

Brand	Thermo Fisher Scientific
Product Type	Master Mix
Pack Size	10 x 5 mL
CAT No	A46112
Application	Molecular Biology

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dyeabased indicators for accurate reaction set up. Coupled with user-supplied primer sets and template, PowerTrack SYBR Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.

Dual Hot-Start Technology for Reliable qPCR

PowerTrack SYBR Green Master Mix features a unique dual hot-start DNA polymerase system, minimizing non-specific amplification and primer-dimer formation. This results in increased specificity and reproducibility, making the master mix ideal for gene expression analysis and other quantitative PCR applications. The integration of both high sensitivity and dual hot-start ensures robust detection, even for low-abundance targets in complex samples.

Universal Compatibility and Ease of Use

Designed for seamless integration with all Applied Biosystems qPCR instruments and compatible with other major qPCR platforms, this master mix provides flexibility in laboratory workflows. Its easy-to-use formulation, complete with a passive ROX reference dye for normalization, streamlines setup and ensures accurate, reproducible results across a wide dynamic range. The reagent is supplied in convenient 2 mL and 5 mL tube formats, supporting various experimental scales.

Superior Consistency and Quality Assurance

Manufactured under ISO 9001 certified processes, each lot of PowerTrack SYBR Green Master Mix undergoes rigorous quality control to guarantee superior performance and consistency. The proprietary formulation maintains activity and fidelity, ensuring reliable results over a 12-month shelf life when stored at -20C. This master mix delivers trusted results for every qPCR application, from research to diagnostics.

FAQ's of Thermo Fisher Scientific Applied Biosystems PowerTrack SYBR Green Master Mix for qPCR:

Q: How should the Applied Biosystems PowerTrack SYBR Green Master Mix be stored to maintain optimal activity?

A: The master mix should be stored at -20C and protected from light to preserve the integrity of its components. Avoid repeated freeze-thaw cycles by aliquoting the reagent upon first use, as this helps maintain consistent performance throughout its 12-month shelf life.

Q: What types of samples are compatible with PowerTrack SYBR Green Master Mix for qPCR?

A: This master mix is validated for a wide range of sample types, including genomic DNA, cDNA, plasmid DNA, and PCR amplicons. Its high inhibitor tolerance ensures reliable amplification and detection even from complex biological samples.

Q: When is it beneficial to use this mix over other qPCR master mixes?

A: You should use PowerTrack SYBR Green Master Mix when you require high sensitivity, superior specificity, and minimal risk of primer-dimer formation-particularly in gene expression studies, copy number variation analysis, or quantification from challenging samples. It is especially useful when you need lot-to-lot consistency and compatibility with passive ROX normalization.

Q: What is the recommended reaction volume range for using this master mix?

A: The master mix is optimized for reaction volumes between 10 L and 50 L, providing flexibility to accommodate a broad range of qPCR applications, from low- to high-throughput experiments.

Q: How does the passive ROX reference dye improve qPCR results in this master mix?

A: The included passive ROX reference dye normalizes fluorescence variations resulting from pipetting or instrument differences, allowing for accurate signal quantification and consistent reproducibility across all major real-time PCR instruments that support ROX normalization.

Q: Where can the PowerTrack SYBR Green Master Mix be used?

A: This reagent is suitable for use in any laboratory setting equipped with compatible real-time PCR thermal cyclers, including Applied Biosystems 7500, QuantStudio, StepOnePlus, and other major qPCR platforms. Its portable 2 mL and 5 mL formats facilitate easy handling in research, diagnostic, or core facilities.

Q: What are the primary benefits of using PowerTrack SYBR Green Master Mix for quantitative PCR applications?

A: Key advantages include high specificity from dual hot-start technology, wide dynamic range for both low and high-abundance targets, robust inhibitor tolerance for complex samples, and reliable quantification with ROX normalization-all contributing to accurate, reproducible, and consistent qPCR results.

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