Product Description
Brand Qiagen
Product Type PCR Kit
Pack Size 800 x 25 ?l
CAT No 206045
Application Molecular Reagent
The Type-it Fast SNP Probe PCR Kit is based on highly specific HotStarTaqPlusDNA Polymerase and a newly developed buffer system, both of which enable reliable and clear allelic discrimination. The combination of all components provided in the master mix result in improved accuracy and highly specific probe binding. The Type-it Fast SNP Probe PCR Kit is validated using TaqMan SNP Genotyping Assays and enables reproducible SNP genotyping even with difficult SNP loci (e.g., GC rich) or low amounts of starting template.
Exceptional Speed and FlexibilityAchieve SNP genotyping results in as little as 45 minutes with the Type-it Fast SNP Probe PCR Kit, designed for both manual and automated PCR workflows. The ready-to-use master mix ensures streamlined setup, supporting multiplexing and high-throughput demands in genomics laboratories. With optimized performance on leading real-time PCR systems, this kit fits seamlessly into existing workflows.
Optimized for High-Throughput LaboratoriesThe kit's robust buffer system enables accurate multiplex SNP analysis, even with a high number of samples. Its compatibility with various types of genomic DNA and adaptability across major real-time PCR cyclers makes it particularly valuable for diagnostic centers, research facilities, and core labs needing quick, scalable variant analysis.
FAQ's of Qiagen Type-it Fast SNP Probe PCR Kit (800):
Q: How do I use the Qiagen Type-it Fast SNP Probe PCR Kit for genotyping?
A: Simply combine your genomic DNA sample with the 2x Type-it Fast SNP Probe PCR Master Mix, RNase-Free Water, and specific SNP probes and primers. Run the reaction using standard real-time PCR cycling conditions as detailed in the kit manual. The entire process can be completed in as little as 45 minutes.
Q: What types of real-time PCR platforms are compatible with this kit?
A: The Qiagen Type-it Fast SNP Probe PCR Kit is optimized for seamless use with ABI, Bio-Rad, Roche, and most other major real-time PCR instruments, ensuring broad instrument compatibility in the laboratory.
Q: When should I use this kit for my SNP analysis needs?
A: This kit is ideal for applications requiring fast, accurate genotyping-particularly in studies involving allelic discrimination and variant analysis where high throughput and specificity are critical.
Q: Where should the kit be stored after receiving it?
A: To maintain reagent integrity, store the kit at -15C to -25C immediately after delivery as per the storage guidelines provided in the instruction manual.
Q: What advantages does the Type-it Fast SNP Probe PCR Kit offer compared to standard PCR kits?
A: It provides rapid cycling with total reaction times as low as 45 minutes and exceptional specificity, making it well-suited for multiplexed SNP genotyping and variant analysis under demanding laboratory conditions.
Q: How is the kit supplied and what are the included components?
A: Each kit contains a 2x Type-it Fast SNP Probe PCR Master Mix, RNase-Free Water, and a detailed instructions manual. This ensures users have all necessary reagents for up to 800 reactions (based on a 25 l volume).
Q: Is the Qiagen Type-it Fast SNP Probe PCR Kit safe to use in a typical laboratory setting?
A: Yes, the kit is classified as non-hazardous under normal laboratory usage. Standard laboratory safety procedures are sufficient.