Agilent Technologies 1290 Infinity III Bio LC System

Agilent Technologies 1290 Infinity III Bio LC System

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Product Details:

Voltage 100240 VAC
Measurement Range Wide range for peptide, protein and nucleic acid separations
Feature Highest UHPLC performance; bio-inert for biocompatible applications; low carryover
Model No 1290 Infinity III Bio LC System
Core Components Bio-inert pump, thermostatted autosampler, column compartment, diode array detector
Power Source AC Mains
Frequency 50/60 Hz
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Agilent Technologies 1290 Infinity III Bio LC System Price And Quantity

Agilent Technologies 1290 Infinity III Bio LC System Product Specifications

Display Type Color touch screen front panel
Accuracy Flow precision <0.07% RSD
Automation Grade Fully automated
Temperature Range 4C to 70C (thermostatted autosampler and column compartment)
Capacity Standard analytical to semi-preparative Configurations, up to 2 mL/min
Frequency 50/60 Hz
Equipment Materials Bio-inert materials (PEEK, titanium, ceramics)
Power Source AC Mains
Core Components Bio-inert pump, thermostatted autosampler, column compartment, diode array detector
Model No 1290 Infinity III Bio LC System
Usage Protein, antibody, and oligonucleotide analysis; biopharmaceutical R&D
Dimension (L*W*H) 420 mm x 600 mm x 620 mm (stacked configuration, varies per modules)
Weight Approximately 35-40 kg (system module dependent)
Feature Highest UHPLC performance; bio-inert for biocompatible applications; low carryover
Measurement Range Wide range for peptide, protein and nucleic acid separations
Voltage 100240 VAC
Type Bio LC System, High-Performance Liquid Chromatography (HPLC)

Software Agilent OpenLab CDS integration
Detectors Available Diode array, multi-wavelength UV, fluorescence, RID
Connectivity LAN, USB ports, RS232
Noise Level < 5 AU
pH Range 1 to 13
Injection Volume Range 0.1 to 100 L
Operating Modes Gradient and isocratic operation
Maximum Pressure 1300 bar (18,800 psi)
Flow Rate Range 0.001 to 2 mL/min (analytical); up to 5 mL/min (semi-preparative)
Delay Volume < 380 L (standard configuration)
Sample Capacity Up to 216 vials or microtiter plates

Product Description

Brand Agilent Technologies

Model 1290

Capacity up to 1300 bar

CAT No 1290

Application Chromatography

The 1290 Infinity III Bio LC System is a UHPLC, binary or quaternary, consisting of biocompatible material for use in biopharma (e.g., critical quality attributes, such as Bio SEC) and other applications utilizing high salt and extreme pH conditions. Biocompatibility ensures the integrity of your biomolecules and robustness of the system.

Engineered for Bio-Inert Separations

This system is optimized with bio-inert materials like PEEK, titanium, and ceramics, making it ideal for sensitive bioanalytical separations of proteins and nucleic acids. Reducing metal-induced sample adsorption ensures accurate and reproducible results for biopharmaceutical research.

Flexible Sample Handling and Throughput

Accommodating up to 216 vials or microtiter plates, the autosampler operates across an injection range of 0.1 to 100 L. This flexible capacity caters to high-throughput labs, while the thermostatted compartment ensures sample integrity and precise temperature control from 4C to 70C.

Advanced Detection and Data Management

The Agilent 1290 Infinity III Bio LC offers multiple detector options including diode array, UV, fluorescence, and RID. Integrated with Agilent OpenLab CDS, it provides comprehensive data acquisition, streamlined analysis, and robust connectivity for laboratory networks.

FAQ's of Agilent Technologies 1290 Infinity III Bio LC System:

Q: How does the 1290 Infinity III Bio LC System ensure sample integrity during protein and oligonucleotide analysis?

A: The system uses bio-inert materials-PEEK, titanium, and ceramics-to minimize sample interaction with metal surfaces, safeguarding sensitive biomolecules. It also features a thermostatted autosampler and column compartment, maintaining temperatures from 4C to 70C for optimal sample preservation.

Q: What types of detectors are available with this HPLC system and how are they beneficial?

A: You can equip the 1290 Infinity III Bio LC System with diode array, multi-wavelength UV, fluorescence, or refractive index detectors. This diversity allows users to tailor detection sensitivity and selectivity to their specific analytical needs, from protein quantification to fluorescent tagging.

Q: When should I use isocratic versus gradient operation modes with this system?

A: Isocratic mode is best for simple separations with minimal matrix complexity, while gradient operation is preferred for complex mixtures such as multi-component biopharmaceutical samples, allowing for enhanced resolution and efficiency.

Q: Where can this system be effectively integrated within a laboratory environment?

A: Thanks to its compact, modular design (420 mm 600 mm 620 mm, stacked), LAN/USB/RS232 connectivity, and compatibility with Agilent OpenLab CDS, the system can be seamlessly implemented in R&D, QA/QC, or process development labs.

Q: What is the process for automated sample injection?

A: The fully automated thermostatted autosampler can handle injection volumes from 0.1 to 100 L and accommodates up to 216 vials or microtiter plates, enabling unattended, high-throughput sample analysis with minimal carryover.

Q: How does the system support regulatory compliance and data security?

A: By integrating with Agilent OpenLab CDS software, the system provides robust electronic data management, audit trails, and user access control, assisting laboratories in meeting regulatory requirements for data integrity and compliance.

Q: What are the main benefits of using the 1290 Infinity III Bio LC System for biopharmaceutical research?

A: Users gain from outstanding UHPLC performance, broad pH compatibility (1 to 13), precise flow control, high throughput, automation, and minimal sample carryover, all essential for reliable protein, antibody, and oligonucleotide analyses.

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