BioRad SsoFast EvaGreen Supermix, 200 x 20 l rxns, 2 ml (2 x 1 ml)

BioRad SsoFast EvaGreen Supermix, 200 x 20 l rxns, 2 ml (2 x 1 ml)

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Product Details:

Measurement Range Wide dynamic range for DNA quantification
Accuracy High; enables precise quantification of nucleic acids
Shelf Life 1 year (when stored as recommended)
Instruments Type Compatible with most real-time PCR instruments
Features High sensitivity, fast cycling capability, compatible with all real-time PCR platforms
Function DNA amplification and real-time detection in PCR
Usage Type Laboratory
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BioRad SsoFast EvaGreen Supermix, 200 x 20 l rxns, 2 ml (2 x 1 ml) Price And Quantity

BioRad SsoFast EvaGreen Supermix, 200 x 20 l rxns, 2 ml (2 x 1 ml) Product Specifications

Condition New
Usage Type Laboratory
Portable Yes
Technology EvaGreen-based quantitative PCR (qPCR)
Dimension (L*W*H) 2 x 1 ml vials (total 2 ml)
Material Reagent Solution (Contains EvaGreen dye, dNTPs, Sso7d-fusion polymerase, MgCl2, buffer)
Operating Type Manual use in qPCR thermal cyclers
Storage Instructions Store at -20C. Avoid repeated freeze-thaw cycles.
Features High sensitivity, fast cycling capability, compatible with all real-time PCR platforms
Instruments Type Compatible with most real-time PCR instruments
Shelf Life 1 year (when stored as recommended)
Color Clear/Pale (liquid)
Accuracy High; enables precise quantification of nucleic acids
Wall Mounted No
Measurement Range Wide dynamic range for DNA quantification
Use For real-time PCR amplification and detection assays
Function DNA amplification and real-time detection in PCR
Real-Time Operation Yes
Equipment Type qPCR Supermix / PCR Reagent

Product Description

Brand BioRad

Product Type PCR Kit

Pack Size 2 mL

CAT No 1725200

Application Molecular Biology

SsoFast EvaGreen Supermix for effective performance in a wide range of qPCR applications. Our patented Sso7d fusion polymerase* uses the Sso7d DNA-binding protein to stabilize the polymerase-template complex, increasing processivity and providing greater speed and reduced reaction times compared to conventional DNA polymerases, without affecting PCR sensitivity, efficiency, or reproducibility.

High-Performance qPCR Reagent

Engineered for speed and sensitivity, SsoFast EvaGreen Supermix ensures robust DNA amplification and real-time detection. Its proprietary buffer, EvaGreen dye, and Sso7d-fusion polymerase provide consistent, reliable results across a wide dynamic range.

Universal Compatibility

Compatible with all leading real-time PCR cyclers, including Bio-Rad, ABI, and Roche, this supermix offers hassle-free integration into a variety of laboratory workflows. Its versatile formula supports both SYBR Green and EvaGreen chemistry assays.

Convenient Storage and Usage

Packaged in two easy-to-handle 1 ml vials, this supermix simplifies setup for up to 200 reactions. Store at -20C and avoid repeated freeze-thaw cycles to sustain optimal reagent quality throughout its one-year shelf life.

FAQ's of BioRad SsoFast EvaGreen Supermix, 200 x 20 Al rxns, 2 ml (2 x 1 ml):

Q: How do I use the BioRad SsoFast EvaGreen Supermix in qPCR reactions?

A: Simply mix the supermix with your DNA template and primers according to your reaction volume (20 l typical), then load into your compatible real-time PCR cycler. Follow the recommended ultra-fast cycling protocols for best results.

Q: What benefits does the SsoFast EvaGreen Supermix offer for real-time PCR?

A: This supermix provides high sensitivity, rapid cycling, and precise DNA quantification. Its optimized formulation improves amplification efficiency and ensures reliable results across a broad dynamic range.

Q: When should I store the supermix at -20C, and how do I avoid degradation?

A: Store the supermix immediately at -20C upon receipt. To prevent loss of activity, avoid repeated freeze-thaw cycles by aliquoting as needed and returning unused portions promptly to cold storage.

Q: What type of instruments is this supermix compatible with?

A: It is compatible with nearly all major real-time PCR cyclers, including Bio-Rad, ABI, and Roche models, making it suitable for most laboratory setups focused on qPCR assays.

Q: Where can I use the SsoFast EvaGreen Supermix?

A: This reagent is designed for use in laboratory environments requiring real-time PCR amplification and detection, such as research, clinical diagnostics, and molecular biology studies.

Q: What is the process for preparing a qPCR reaction with this supermix?

A: Combine supermix, template DNA, and primers, then mix and briefly centrifuge. Load reaction mixtures into qPCR tubes or plates and initiate run on your compatible thermal cycler, following the recommended fast-cycling protocol.

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