Product Description
Brand
Qiagen
Sample Type
Whole Blood
Pack Size
96 Wells
CAT No
622180
QuantiFERON-TB Gold Plus (QFT-Plus) assesses the cell-mediated immune (CMI) response to TB-specific ESAT-6 and CFP-10 antigens through a quantitative measurement of IFN-I3 in a whole blood sample. QFT-Plus is an indirect test for M. tuberculosis infection (including disease) and is intended for use in conjunction with risk assessment, radiography, and other medical and diagnostic evaluations.
Comprehensive Solution for TB DetectionThe QuantiFERON-TB Gold Plus 2 Plate ELISA Kit provides a reliable assay for the detection of tuberculosis infection. Designed for laboratory use, it delivers consistent results using whole blood samples with a straightforward manual protocol. The kit supports a complete workflow with all necessary reagents included, offering high sensitivity and specificity for clinical and research applications.
Highly Sensitive Sandwich ELISA TechnologyUtilizing advanced sandwich ELISA technology, this kit enables robust detection of IFN- released in response to Mycobacterium tuberculosis antigens. The colorimetric readout at 450 nm ensures accurate quantification over a 0-10 IU/mL range. The high and low controls facilitate reliable test interpretation and laboratory quality assurance.
Convenient and Scalable for Laboratory NeedsWith capacity for up to 192 determinations, this kit serves the needs of both routine and high-throughput laboratories. Its easy storage at 2C to 8C and straightforward assay process make it suitable for dealers, distributors, exporters, manufacturers, suppliers, traders, and wholesalers operating under India's regulatory frameworks.
FAQ's of Qiagen QuantiFERON-TB Gold Plus 2 Plate ELISA Kit:
Q: How do I use the QuantiFERON-TB Gold Plus 2 Plate ELISA Kit for TB diagnosis?
A: This kit is used by collecting 1 mL of human whole blood per tube, stimulating cells with TB-specific antigens, then incubating for approximately 16-24 hours. The resulting IFN- is measured using the included reagents in a manual sandwich ELISA format, with a colorimetric readout at 450 nm.
Q: What sample type is required and how much volume should I collect?
A: The assay uses human whole blood, with 1 mL required per tube. Ensure proper collection and handling to maintain sample integrity for reliable interferon-gamma detection.
Q: When should the kit be used in the testing process?
A: The kit should be used after whole blood collection and antigen stimulation, following the incubation period of 16-24 hours. It is designed for laboratory-based analysis and not for point-of-care testing.
Q: Where can the QuantiFERON-TB Gold Plus kit be stored, and under what conditions?
A: All kit contents must be stored at 2C to 8C as specified on the product label. This ensures reagent stability and consistent assay performance until the marked expiry date.
Q: What is the process for analyzing results using this kit?
A: After the incubation and ELISA procedure, read plates at 450 nm using a microplate reader. Compare sample signal intensities to the standard curve (0-10 IU/mL IFN-) and interpret results using the high and low controls provided.
Q: How does this kit benefit clinical and research laboratories?
A: Its high sensitivity and specificity enable accurate TB infection detection, supporting reliable diagnosis and research outcomes. The CE-marked IVD status ensures quality and regulatory compliance in diverse laboratory environments.
Q: What is included in the 2 Plate format and how many tests can be performed?
A: The kit includes 2 x 96-well plates, conjugate, substrate, standards, high and low controls, and sample diluent, supporting up to 192 individual determinations, making it suitable for medium- to high-throughput laboratory testing.